Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 316428..317228 Replicon chromosome
Accession NZ_CP101257
Organism Escherichia coli strain EC21Z-078

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NML20_RS01460 Protein ID WP_000342449.1
Coordinates 316701..317228 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NML20_RS01455 Protein ID WP_001277108.1
Coordinates 316428..316694 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML20_RS01435 (312085) 312085..312753 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NML20_RS01440 (312746) 312746..313804 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NML20_RS01445 (314049) 314049..314903 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NML20_RS01450 (315175) 315175..316278 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NML20_RS01455 (316428) 316428..316694 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NML20_RS01460 (316701) 316701..317228 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NML20_RS01465 (317225) 317225..317608 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NML20_RS01470 (318032) 318032..319141 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NML20_RS01475 (319189) 319189..320115 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NML20_RS01480 (320112) 320112..321389 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NML20_RS01485 (321386) 321386..322153 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T251679 WP_000342449.1 NZ_CP101257:316701-317228 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT251679 WP_001277108.1 NZ_CP101257:316428-316694 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References