Detailed information of TA system TA025165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3916404..3917434
• Replicon: chromosome
• Accession: NC_015259
• Organism: Polymorphum gilvum SL003B-26A1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: SL003B_RS18240
• Protein ID: WP_013654348.1
• Coordinates: 3916404..3916973 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: F2J1X9
• Locus tag: SL003B_RS18245
• Protein ID: WP_013654349.1
• Coordinates: 3916970..3917434 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SL003B_RS23430	3912053..3912535	+	483	WP_148259342.1	DUF1566 domain-containing protein	-
SL003B_RS22465	3912957..3913280	+	324	WP_049792613.1	hypothetical protein	-
SL003B_RS18205	3913281..3913880	+	600	WP_013654341.1	recombinase family protein	-
SL003B_RS18210	3913864..3914091	+	228	WP_013654342.1	WGR domain-containing protein	-
SL003B_RS18215	3914156..3914656	-	501	WP_041375629.1	hypothetical protein	-
SL003B_RS18220	3914869..3915288	+	420	WP_148259344.1	hypothetical protein	-
SL003B_RS18225	3915373..3915606	-	234	WP_013654345.1	Arm DNA-binding domain-containing protein	-
SL003B_RS18230	3915603..3915830	-	228	WP_013654346.1	hypothetical protein	-
SL003B_RS18235	3915827..3916186	-	360	WP_013654347.1	DUF2958 domain-containing protein	-
SL003B_RS18240	3916404..3916973	-	570	WP_013654348.1	PIN domain-containing protein	Toxin
SL003B_RS18245	3916970..3917434	-	465	WP_013654349.1	helix-turn-helix domain-containing protein	Antitoxin
SL003B_RS18250	3917755..3917961	+	207	WP_041375630.1	hypothetical protein	-
SL003B_RS18255	3917988..3918893	-	906	WP_013654350.1	DUF1738 domain-containing protein	-
SL003B_RS18260	3919206..3920099	-	894	WP_013654351.1	abortive infection family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3892502..3926505	34003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Helix-turn-helix domain, group 17 (81-126)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 20960.19 Da
• Isoelectric Point: 5.5636

>T25165 WP_013654348.1 NC_015259:c3916973-3916404 [Polymorphum gilvum SL003B-26A1]
MSRYAALLDANVLYPAPLRDLLLQLAVTDLFRAKWTADIHREWIEALLRNEPMRDRAALERTRDLMDRATRDSLVEGYHA
LIPALTLPDADDRHVLAAAIVGRCDVIVTQNLADFPAEALDPFGIDVQHPDQFLVNHLHLAPGLFCACVRKVRARLKNPP
YSVEAYLATLTQVGLVATAGELGGFADLL

Nucleotide

• Length: 570 bp

>T25165 NC_015259:c3916973-3916404 [Polymorphum gilvum SL003B-26A1]
ATGAGCCGCTACGCCGCCCTGCTTGATGCCAATGTCCTCTATCCCGCGCCGTTGCGGGACTTGCTCTTGCAGCTTGCCGT
GACCGACCTGTTCAGGGCGAAATGGACGGCGGACATTCATCGGGAATGGATCGAGGCGCTGCTCCGCAACGAGCCGATGC
GAGACCGCGCCGCATTGGAACGCACCCGCGACCTCATGGATCGGGCTACGCGCGACAGCCTGGTCGAAGGCTATCATGCC
CTGATCCCGGCACTGACGCTGCCGGACGCCGACGACCGCCATGTGCTGGCCGCCGCGATTGTCGGCCGCTGCGACGTGAT
CGTGACGCAGAACCTCGCCGACTTTCCTGCCGAAGCTCTGGACCCGTTCGGCATCGACGTGCAGCACCCCGACCAGTTTC
TGGTCAACCATCTGCATCTCGCGCCGGGCCTCTTCTGCGCTTGTGTCCGAAAGGTCCGCGCCCGGCTCAAGAACCCGCCC
TACAGTGTGGAAGCCTATCTCGCCACCTTGACGCAGGTGGGGCTTGTCGCCACGGCGGGCGAACTCGGCGGCTTTGCCGA
TCTTCTCTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17096.79 Da
• Isoelectric Point: 7.3203

>AT25165 WP_013654349.1 NC_015259:c3917434-3916970 [Polymorphum gilvum SL003B-26A1]
MTMLAHRQLPPSAQDAAIARVSGQLLSRYARQKRPLTLRVTDADQERPIELPAGAVALLMDILEAMAAGRGVTLMPESAE
LTTVQAAEVLNVSRPFLIKLLDDGTIPHRKVGKHRRIRMEDVMAYKTAIDREREAVLDQLVSEAQEQDMGYGRP

Nucleotide

• Length: 465 bp

>AT25165 NC_015259:c3917434-3916970 [Polymorphum gilvum SL003B-26A1]
ATGACCATGCTCGCACATCGACAACTCCCGCCCTCGGCGCAGGATGCCGCGATCGCGCGCGTGTCCGGCCAACTTCTATC
CCGCTATGCGCGCCAGAAGCGGCCGTTGACGCTGCGCGTCACCGACGCCGATCAGGAGCGGCCCATAGAGCTGCCGGCCG
GCGCGGTCGCGCTGCTGATGGACATCCTCGAAGCGATGGCCGCCGGCCGTGGCGTCACCCTCATGCCGGAGAGCGCCGAG
CTGACGACGGTGCAGGCCGCCGAAGTGCTCAACGTCTCCCGGCCGTTCCTTATCAAGCTGCTGGACGATGGCACGATCCC
GCATCGCAAGGTCGGCAAGCACCGGCGCATCCGCATGGAAGACGTGATGGCCTACAAGACGGCCATCGACCGCGAACGCG
AAGCGGTGCTCGACCAGCTTGTCAGCGAGGCGCAGGAACAGGACATGGGCTATGGCCGCCCATGA

Structures

Antitoxin

• AlphaFold DB: F2J1X9