Detailed information of TA system TA025164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 1481071..1481600
• Replicon: chromosome
• Accession: NC_015259
• Organism: Polymorphum gilvum SL003B-26A1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: F2J2A4
• Locus tag: SL003B_RS06950
• Protein ID: WP_013652117.1
• Coordinates: 1481310..1481600 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F2J2A3
• Locus tag: SL003B_RS06945
• Protein ID: WP_013652116.1
• Coordinates: 1481071..1481307 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SL003B_RS06925	1478231..1478452	-	222	WP_013652113.1	ribbon-helix-helix domain-containing protein	-
SL003B_RS06930	1478449..1478649	-	201	WP_041375417.1	DUF4169 family protein	-
SL003B_RS06935	1478673..1479281	-	609	WP_013652114.1	SspB family protein	-
SL003B_RS06940	1479906..1480865	+	960	WP_193371738.1	thymidylate synthase	-
SL003B_RS06945	1481071..1481307	+	237	WP_013652116.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
SL003B_RS06950	1481310..1481600	+	291	WP_013652117.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SL003B_RS22715	1481625..1481747	+	123	Protein_1384	thymidylate synthase	-
SL003B_RS06955	1481901..1482473	+	573	WP_013652118.1	TRAP transporter small permease subunit	-
SL003B_RS06960	1482473..1483858	+	1386	WP_013652119.1	TRAP transporter large permease subunit	-
SL003B_RS06965	1484151..1485245	-	1095	WP_041375419.1	TRAP transporter substrate-binding protein	-
SL003B_RS06970	1485413..1486123	+	711	WP_083812063.1	dihydrofolate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10816.40 Da
• Isoelectric Point: 8.0532

>T25164 WP_013652117.1 NC_015259:1481310-1481600 [Polymorphum gilvum SL003B-26A1]
MRRIQFSPAAEADLDAIWAYTLGQWGEAQAIRYFRDMQATCAGLASGTLSGRSVDDVRPGYFKAAFGAHLLFYRLTEEAL
VVIRVLHQRMDVARHL

Nucleotide

• Length: 291 bp

>T25164 NC_015259:1481310-1481600 [Polymorphum gilvum SL003B-26A1]
ATGCGGCGGATCCAGTTCTCGCCGGCAGCGGAAGCGGATCTCGATGCCATCTGGGCTTATACTCTCGGGCAATGGGGCGA
AGCGCAGGCGATCCGATATTTCCGCGACATGCAGGCGACTTGCGCGGGTCTGGCATCCGGGACGCTTTCCGGGCGGTCCG
TCGATGATGTTCGACCCGGATATTTCAAGGCTGCTTTCGGAGCCCATCTGCTCTTCTACCGCCTCACGGAAGAGGCCCTG
GTCGTGATCCGGGTCCTGCACCAGAGGATGGATGTCGCCCGTCACCTATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8601.46 Da
• Isoelectric Point: 4.8007

>AT25164 WP_013652116.1 NC_015259:1481071-1481307 [Polymorphum gilvum SL003B-26A1]
MARNTSISLGDHFADFVDQRVKTGRYGSASDVVRAGLRLLEEHETRMDALRAALEAGEASGEPKDFDFDTFLAHKTSD

Nucleotide

• Length: 237 bp

>AT25164 NC_015259:1481071-1481307 [Polymorphum gilvum SL003B-26A1]
ATGGCGCGCAACACCTCGATTTCCCTAGGCGATCATTTCGCCGATTTCGTCGATCAAAGGGTCAAGACCGGGCGCTACGG
CAGCGCGAGCGACGTGGTGCGGGCGGGACTGCGCCTGCTTGAAGAGCACGAAACACGGATGGACGCCCTCAGGGCAGCCC
TGGAGGCCGGCGAAGCGTCCGGCGAGCCGAAGGATTTCGATTTCGACACATTTCTCGCCCACAAGACGAGCGACTAG

Structures

Toxin

• AlphaFold DB: F2J2A4

Antitoxin

• AlphaFold DB: F2J2A3