Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PanAT/DUF4065(antitoxin)
Location 4284151..4285195 Replicon chromosome
Accession NZ_CP101245
Organism Escherichia coli strain EC21Z-097

Toxin (Protein)


Gene name panT Uniprot ID A0A829CP20
Locus tag NML22_RS20895 Protein ID WP_000019186.1
Coordinates 4284151..4284699 (-) Length 183 a.a.

Antitoxin (Protein)


Gene name panA Uniprot ID A0A839B6W4
Locus tag NML22_RS20900 Protein ID WP_000287252.1
Coordinates 4284722..4285195 (-) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML22_RS20855 (4279475) 4279475..4279540 - 66 Protein_4074 phage minor tail protein L -
NML22_RS20860 (4279540) 4279540..4279914 - 375 Protein_4075 hypothetical protein -
NML22_RS20865 (4279958) 4279958..4280158 - 201 WP_000649477.1 YdaS family helix-turn-helix protein -
NML22_RS20870 (4280249) 4280249..4280923 + 675 WP_000859462.1 LexA family transcriptional repressor -
NML22_RS20875 (4281590) 4281590..4281952 + 363 WP_000135680.1 protein YfdP -
NML22_RS20880 (4282018) 4282018..4282842 + 825 WP_001753751.1 DUF2303 family protein -
NML22_RS20885 (4283029) 4283029..4283811 + 783 WP_000610754.1 hypothetical protein -
NML22_RS20890 (4283848) 4283848..4284117 + 270 WP_001093912.1 pyocin activator PrtN family protein -
NML22_RS20895 (4284151) 4284151..4284699 - 549 WP_000019186.1 hypothetical protein Toxin
NML22_RS20900 (4284722) 4284722..4285195 - 474 WP_000287252.1 DUF4065 domain-containing protein Antitoxin
NML22_RS20905 (4285500) 4285500..4285604 - 105 Protein_4084 tRNA-dihydrouridine synthase -
NML22_RS20910 (4285967) 4285967..4286983 - 1017 WP_000912595.1 DUF2713 family protein -
NML22_RS20915 (4287301) 4287301..4287816 + 516 WP_001295691.1 zinc uptake transcriptional repressor Zur -
NML22_RS20920 (4287858) 4287858..4288067 - 210 WP_001030593.1 CsbD family protein -
NML22_RS20925 (4288183) 4288183..4289508 - 1326 WP_001301116.1 MATE family efflux transporter DinF -
NML22_RS20930 (4289581) 4289581..4290189 - 609 WP_000646078.1 transcriptional repressor LexA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4268559..4290189 21630
- inside Prophage - - 4268559..4290667 22108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Antitoxin SocA-like, Panacea domain

(27-123)


Sequences

Toxin        


Download         Length: 183 a.a.        Molecular weight: 20026.39 Da        Isoelectric Point: 4.4481

>T251635 WP_000019186.1 NZ_CP101245:c4284699-4284151 [Escherichia coli]
MSHNSDIYKLIGAAAGVENGRSEASLNSTDSQEQAFESAFEPSNHERDDDSSSENKAILEEEEFGSNTGALHEFMQQNRM
DSLQAQLDMLKSQVRDKIADATGKEIDNELRTKMASFTVWFMSCWCLFVVAMFTSFLIAHEGKAPVEAIVALLGTSTISI
VGLVGFVVSGLFKSRKDSDKEK

Download         Length: 549 bp

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17298.80 Da        Isoelectric Point: 8.4143

>AT251635 WP_000287252.1 NZ_CP101245:c4285195-4284722 [Escherichia coli]
MYSPVQIANKFITLGNQHHNPLTHMQLQKLTYIAHGYYLALTGKPLLNECVSAWKYGPVIPGMYDAFKDYGNKPVTNVAV
APFGGIVTMDPQAESIIGAVYKFYGSKNGIELSTLTHMPGTPWSQAYNGIGSSIISNDAIKAYYHDLLNNRQQCQGL

Download         Length: 474 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CP20

Antitoxin

Source ID Structure
AlphaFold DB A0A839B6W4

References