Detailed information of TA system TA025161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 734795..735372
• Replicon: chromosome
• Accession: NC_015259
• Organism: Polymorphum gilvum SL003B-26A1

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SL003B_RS03540
• Protein ID: WP_013651447.1
• Coordinates: 734795..735127 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: F2IUS1
• Locus tag: SL003B_RS03545
• Protein ID: WP_013651448.1
• Coordinates: 735121..735372 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SL003B_RS03515	730228..730437	+	210	WP_148259247.1	hypothetical protein	-
SL003B_RS03520	731069..731545	+	477	WP_148259387.1	hypothetical protein	-
SL003B_RS03525	731628..733232	+	1605	WP_148259386.1	relaxase/mobilization nuclease domain-containing protein	-
SL003B_RS03530	733316..733933	+	618	WP_013651444.1	plasmid pRiA4b ORF-3 family protein	-
SL003B_RS23250	733975..734307	-	333	WP_148259248.1	hypothetical protein	-
SL003B_RS23255	734386..734772	+	387	WP_013651446.1	hypothetical protein	-
SL003B_RS03540	734795..735127	-	333	WP_013651447.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SL003B_RS03545	735121..735372	-	252	WP_013651448.1	MazE family transcriptional regulator	Antitoxin
SL003B_RS03550	735452..735811	-	360	WP_013651449.1	hypothetical protein	-
SL003B_RS03555	735827..737293	-	1467	WP_148259388.1	type IV secretory system conjugative DNA transfer family protein	-
SL003B_RS03560	737697..737924	-	228	WP_041375363.1	hypothetical protein	-
SL003B_RS03565	737926..738498	-	573	WP_041375803.1	ParA family protein	-
SL003B_RS03575	738556..739505	+	950	WP_085997565.1	IS630 family transposase	-
SL003B_RS23260	739477..739635	-	159	WP_193371722.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	698692..751055	52363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12775.91 Da
• Isoelectric Point: 10.0996

>T25161 WP_013651447.1 NC_015259:c735127-734795 [Polymorphum gilvum SL003B-26A1]
VVADVRRYDVYLVNLDPTLGSEIKKTRPCLVISPDEMNRHIRTVIIAPMTSVRRDYPSRVDVTFQRKKGQVVLDQIRTVD
KARLAKHLGRLPEPRCREVADLLQQMFAYE

Nucleotide

• Length: 333 bp

>T25161 NC_015259:c735127-734795 [Polymorphum gilvum SL003B-26A1]
GTGGTAGCGGATGTCCGGCGTTATGACGTGTATCTGGTGAATCTCGACCCCACACTGGGGTCAGAGATCAAGAAGACCCG
GCCTTGCCTGGTCATCTCGCCGGACGAGATGAACCGGCACATCCGGACGGTCATCATCGCTCCCATGACTTCCGTGCGGC
GGGACTACCCGAGCCGCGTCGATGTCACCTTCCAGCGCAAGAAAGGACAAGTGGTTCTCGACCAGATACGGACAGTGGAC
AAGGCGCGCCTCGCCAAGCATCTCGGACGCCTCCCGGAGCCGCGTTGTCGCGAGGTCGCCGACCTTCTTCAGCAGATGTT
CGCCTACGAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9321.65 Da
• Isoelectric Point: 4.8181

>AT25161 WP_013651448.1 NC_015259:c735372-735121 [Polymorphum gilvum SL003B-26A1]
MKVNLVPIGNSKGVRIPSSVIKECGFGEQIEMRVEHGIVVLAPARRTRDGWDGAFAKMAAAKDDALLVPDTMDHDWDKEE
WEW

Nucleotide

• Length: 252 bp

>AT25161 NC_015259:c735372-735121 [Polymorphum gilvum SL003B-26A1]
ATGAAAGTGAATCTGGTTCCGATCGGCAATTCCAAGGGCGTGCGGATTCCAAGCTCGGTCATCAAGGAATGCGGCTTCGG
CGAGCAAATCGAGATGCGCGTCGAGCACGGCATTGTGGTGCTGGCGCCCGCCCGCCGTACTCGCGATGGCTGGGATGGCG
CGTTCGCGAAGATGGCTGCCGCCAAGGACGATGCCCTGCTTGTCCCCGACACGATGGATCATGATTGGGACAAGGAGGAA
TGGGAGTGGTAG

Structures

Antitoxin

• AlphaFold DB: F2IUS1