Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 47131..47929 Replicon plasmid pEC21Z137-115K
Accession NZ_CP101235
Organism Escherichia coli strain EC21Z-137

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag NML24_RS23510 Protein ID WP_089075245.1
Coordinates 47408..47929 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag NML24_RS23505 Protein ID WP_001711191.1
Coordinates 47131..47400 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML24_RS23480 (NML24_23445) 42555..43895 - 1341 WP_023135655.1 DnaB-like helicase C-terminal domain-containing protein -
NML24_RS23485 (NML24_23450) 43956..44681 - 726 WP_087904723.1 hypothetical protein -
NML24_RS23490 (NML24_23455) 44964..45731 + 768 WP_001711193.1 hypothetical protein -
NML24_RS23495 (NML24_23460) 45784..46137 - 354 WP_160378290.1 hypothetical protein -
NML24_RS23500 (NML24_23465) 46143..46811 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
NML24_RS23505 (NML24_23470) 47131..47400 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
NML24_RS23510 (NML24_23475) 47408..47929 + 522 WP_089075245.1 GNAT family N-acetyltransferase Toxin
NML24_RS23515 (NML24_23480) 48098..48349 - 252 WP_254841648.1 hypothetical protein -
NML24_RS23520 (NML24_23485) 48352..49044 - 693 WP_024172707.1 membrane protein -
NML24_RS23525 (NML24_23490) 49058..49381 - 324 WP_001711185.1 hypothetical protein -
NML24_RS23530 (NML24_23495) 49515..50096 - 582 WP_032187799.1 tail fiber assembly protein -
NML24_RS23535 (NML24_23500) 50096..52810 - 2715 WP_254841650.1 tail fiber protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..115200 115200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19368.15 Da        Isoelectric Point: 7.8112

>T251593 WP_089075245.1 NZ_CP101235:47408-47929 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT251593 WP_001711191.1 NZ_CP101235:47131-47400 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References