Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86542..88165 Replicon plasmid pEC21Z137-237K
Accession NZ_CP101234
Organism Escherichia coli strain EC21Z-137

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NML24_RS22415 Protein ID WP_000088046.1
Coordinates 86840..88165 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NML24_RS22410 Protein ID WP_024136327.1
Coordinates 86542..86835 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML24_RS22385 (NML24_22360) 82414..83655 + 1242 WP_001053338.1 VWA domain-containing protein -
NML24_RS22390 (NML24_22365) 84218..84499 - 282 WP_000077926.1 hypothetical protein -
NML24_RS22395 (NML24_22370) 84549..84740 - 192 WP_000398480.1 hypothetical protein -
NML24_RS22400 (NML24_22375) 84832..85173 - 342 WP_001151575.1 hypothetical protein -
NML24_RS22405 (NML24_22380) 85546..85938 + 393 WP_000341066.1 hypothetical protein -
NML24_RS22410 (NML24_22385) 86542..86835 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NML24_RS22415 (NML24_22390) 86840..88165 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NML24_RS22420 (NML24_22395) 88226..88432 + 207 WP_000134171.1 hypothetical protein -
NML24_RS22425 (NML24_22400) 88534..88944 + 411 WP_000985911.1 hypothetical protein -
NML24_RS22430 (NML24_22405) 88957..89772 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NML24_RS22435 (NML24_22410) 90026..90451 + 426 WP_001043843.1 hypothetical protein -
NML24_RS22440 (NML24_22415) 91000..91308 + 309 WP_001224687.1 hypothetical protein -
NML24_RS22445 (NML24_22420) 91330..92181 + 852 WP_225517698.1 HNH endonuclease signature motif containing protein -
NML24_RS22450 (NML24_22425) 92243..92446 + 204 WP_001194555.1 hypothetical protein -
NML24_RS22455 (NML24_22430) 92788..93144 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / floR / aph(6)-Id / tet(A) / sul3 / mph(A) / ARR-3 / dfrA14 - 1..237484 237484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T251591 WP_000088046.1 NZ_CP101234:86840-88165 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT251591 WP_024136327.1 NZ_CP101234:86542-86835 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References