Detailed information of TA system TA025159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 529808..530385
• Replicon: chromosome
• Accession: NC_015259
• Organism: Polymorphum gilvum SL003B-26A1

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SL003B_RS02585
• Protein ID: WP_013651267.1
• Coordinates: 529808..530140 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: F2J3W2
• Locus tag: SL003B_RS02590
• Protein ID: WP_013651268.1
• Coordinates: 530134..530385 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SL003B_RS02560	524903..525268	+	366	WP_013651262.1	hypothetical protein	-
SL003B_RS23500	525543..525707	-	165	WP_158306621.1	hypothetical protein	-
SL003B_RS02565	526015..526310	+	296	Protein_511	hypothetical protein	-
SL003B_RS02570	526929..527372	+	444	WP_148259380.1	hypothetical protein	-
SL003B_RS02575	527362..529059	+	1698	WP_041375331.1	relaxase/mobilization nuclease domain-containing protein	-
SL003B_RS02580	529142..529756	+	615	WP_013651266.1	plasmid pRiA4b ORF-3 family protein	-
SL003B_RS02585	529808..530140	-	333	WP_013651267.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SL003B_RS02590	530134..530385	-	252	WP_013651268.1	MazE family transcriptional regulator	Antitoxin
SL003B_RS02595	530435..530824	-	390	WP_013651269.1	hypothetical protein	-
SL003B_RS02600	530840..532435	-	1596	WP_083812031.1	type IV secretory system conjugative DNA transfer family protein	-
SL003B_RS22210	532797..533531	+	735	WP_083812152.1	LuxR family transcriptional regulator	-
SL003B_RS22605	533634..534245	+	612	WP_193371731.1	conjugal transfer protein TraI	-
SL003B_RS23205	534242..534460	+	219	WP_013651272.1	hypothetical protein	-
SL003B_RS22610	534505..535095	+	591	WP_041375772.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	503101..557329	54228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12685.81 Da
• Isoelectric Point: 10.3102

>T25159 WP_013651267.1 NC_015259:c530140-529808 [Polymorphum gilvum SL003B-26A1]
VVADVRRYDVYLVNLDPTLGSEIKKTRPCLVISPDEMNRHIRTVIIAPMTSVRRDYPSRVDVTFQRKKGQVVLDQIRTVD
KARLAKHLGRLPEPRCREVAGVLQQIFAYE

Nucleotide

• Length: 333 bp

>T25159 NC_015259:c530140-529808 [Polymorphum gilvum SL003B-26A1]
GTGGTAGCGGATGTCCGACGGTACGACGTATATCTGGTAAACCTCGACCCCACGCTGGGGTCGGAAATCAAGAAGACCCG
GCCTTGTCTGGTCATATCGCCGGACGAGATGAATCGGCACATCCGGACGGTCATCATCGCTCCCATGACCTCCGTGCGGC
GGGACTATCCGAGCCGCGTCGATGTCACCTTCCAGCGGAAGAAGGGACAGGTGGTTCTCGACCAGATACGGACAGTGGAC
AAGGCGCGCCTCGCCAAGCATCTCGGGCGCCTGCCGGAGCCGCGTTGTCGCGAGGTCGCCGGCGTTCTTCAGCAGATATT
CGCCTACGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.70 Da
• Isoelectric Point: 4.8307

>AT25159 WP_013651268.1 NC_015259:c530385-530134 [Polymorphum gilvum SL003B-26A1]
MKVNLVPIGNSKGVRIPSSVIKECGFGEQIEMRVEHGIVVLAPARRTRDGWDGAFAKMAAAKDDALLIPDTMEHDWDKEE
WEW

Nucleotide

• Length: 252 bp

>AT25159 NC_015259:c530385-530134 [Polymorphum gilvum SL003B-26A1]
ATGAAAGTGAACCTGGTTCCGATCGGCAATTCCAAGGGCGTGCGGATTCCAAGCTCGGTCATCAAGGAGTGCGGCTTCGG
CGAGCAGATCGAGATGCGCGTCGAGCACGGCATTGTCGTGCTAGCGCCTGCCCGTCGCACCCGCGATGGCTGGGACGGCG
CGTTCGCGAAGATGGCCGCCGCCAAGGACGATGCCCTGCTTATCCCCGATACGATGGAGCATGACTGGGATAAGGAGGAA
TGGGAGTGGTAG

Structures

Antitoxin

• AlphaFold DB: F2J3W2