Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 848516..849170 Replicon chromosome
Accession NZ_CP101233
Organism Escherichia coli strain EC21Z-137

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NML24_RS04215 Protein ID WP_000244781.1
Coordinates 848763..849170 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NML24_RS04210 Protein ID WP_000354046.1
Coordinates 848516..848782 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML24_RS04185 (843685) 843685..844428 + 744 WP_186185804.1 SDR family oxidoreductase -
NML24_RS04190 (844485) 844485..845918 - 1434 WP_032278899.1 6-phospho-beta-glucosidase BglA -
NML24_RS04195 (845963) 845963..846274 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NML24_RS04200 (846438) 846438..847097 + 660 WP_000250274.1 hemolysin III family protein -
NML24_RS04205 (847293) 847293..848273 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
NML24_RS04210 (848516) 848516..848782 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NML24_RS04215 (848763) 848763..849170 + 408 WP_000244781.1 protein YgfX Toxin
NML24_RS04220 (849210) 849210..849731 - 522 WP_001055874.1 flavodoxin FldB -
NML24_RS04225 (849843) 849843..850739 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NML24_RS04230 (850764) 850764..851474 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NML24_RS04235 (851480) 851480..853213 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T251580 WP_000244781.1 NZ_CP101233:848763-849170 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T251580 NZ_CP101233:848763-849170 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCTTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251580 WP_000354046.1 NZ_CP101233:848516-848782 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT251580 NZ_CP101233:848516-848782 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References