Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3950084..3950738 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NML25_RS18890 Protein ID WP_000244781.1
Coordinates 3950084..3950491 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NML25_RS18895 Protein ID WP_000354046.1
Coordinates 3950472..3950738 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS18870 (3946041) 3946041..3947774 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
NML25_RS18875 (3947780) 3947780..3948490 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NML25_RS18880 (3948515) 3948515..3949411 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NML25_RS18885 (3949523) 3949523..3950044 + 522 WP_001055874.1 flavodoxin FldB -
NML25_RS18890 (3950084) 3950084..3950491 - 408 WP_000244781.1 protein YgfX Toxin
NML25_RS18895 (3950472) 3950472..3950738 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NML25_RS18900 (3950981) 3950981..3951961 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
NML25_RS18905 (3952038) 3952038..3952697 - 660 WP_000250274.1 hemolysin III family protein -
NML25_RS18910 (3952861) 3952861..3953172 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
NML25_RS18915 (3953217) 3953217..3954650 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T251559 WP_000244781.1 NZ_CP101222:c3950491-3950084 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251559 WP_000354046.1 NZ_CP101222:c3950738-3950472 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References