Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1851197..1851981 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NML25_RS08670 Protein ID WP_000613626.1
Coordinates 1851197..1851691 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag NML25_RS08675 Protein ID WP_001110447.1
Coordinates 1851688..1851981 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS08650 (1846390) 1846390..1847487 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
NML25_RS08655 (1847487) 1847487..1848428 + 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NML25_RS08660 (1848494) 1848494..1850137 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
NML25_RS08665 (1850149) 1850149..1851102 + 954 WP_001212768.1 flagellar hook-associated protein FlgL -
NML25_RS08670 (1851197) 1851197..1851691 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NML25_RS08675 (1851688) 1851688..1851981 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
NML25_RS08680 (1852114) 1852114..1855299 - 3186 WP_001550951.1 ribonuclease E -
NML25_RS08685 (1855872) 1855872..1856831 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T251549 WP_000613626.1 NZ_CP101222:c1851691-1851197 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT251549 WP_001110447.1 NZ_CP101222:c1851981-1851688 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References