Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1190038..1190656 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag NML25_RS05675 Protein ID WP_001291435.1
Coordinates 1190038..1190256 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag NML25_RS05680 Protein ID WP_000344800.1
Coordinates 1190282..1190656 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS05640 (1185327) 1185327..1185899 + 573 WP_000779831.1 YbaY family lipoprotein -
NML25_RS05645 (1185930) 1185930..1186241 - 312 WP_000409911.1 MGMT family protein -
NML25_RS05655 (1186620) 1186620..1186973 + 354 WP_001550741.1 DUF1428 family protein -
NML25_RS05660 (1187015) 1187015..1188565 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
NML25_RS05665 (1188729) 1188729..1189199 - 471 WP_000136192.1 YlaC family protein -
NML25_RS05670 (1189315) 1189315..1189866 - 552 WP_000102564.1 maltose O-acetyltransferase -
NML25_RS05675 (1190038) 1190038..1190256 - 219 WP_001291435.1 HHA domain-containing protein Toxin
NML25_RS05680 (1190282) 1190282..1190656 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
NML25_RS05685 (1191202) 1191202..1194351 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
NML25_RS05690 (1194374) 1194374..1195567 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T251547 WP_001291435.1 NZ_CP101222:c1190256-1190038 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT251547 WP_000344800.1 NZ_CP101222:c1190656-1190282 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References