Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 293085..293582 Replicon chromosome
Accession NZ_CP101222
Organism Escherichia coli strain EC21Z-144

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NML25_RS01345 Protein ID WP_001173343.1
Coordinates 293409..293582 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NML25_RS01340 Protein ID WP_000398619.1
Coordinates 293085..293381 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML25_RS01320 (289815) 289815..290534 - 720 WP_000611288.1 two-component system response regulator DcuR -
NML25_RS01325 (290531) 290531..292162 - 1632 WP_001216477.1 sensor histidine kinase -
NML25_RS01330 (292342) 292342..292572 + 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NML25_RS01335 (292584) 292584..292856 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NML25_RS01340 (293085) 293085..293381 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NML25_RS01345 (293409) 293409..293582 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NML25_RS01350 (293701) 293701..295218 - 1518 WP_001550472.1 lysine--tRNA ligase -
NML25_RS01355 (295455) 295455..296912 - 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251541 WP_001173343.1 NZ_CP101222:293409-293582 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251541 WP_000398619.1 NZ_CP101222:293085-293381 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References