Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 87319..88942 Replicon plasmid pEC21Z147-274K
Accession NZ_CP101211
Organism Escherichia coli strain EC21Z-147

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NML26_RS25050 Protein ID WP_000088046.1
Coordinates 87617..88942 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NML26_RS25045 Protein ID WP_024136327.1
Coordinates 87319..87612 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML26_RS25020 (NML26_25030) 83191..84432 + 1242 WP_001053338.1 VWA domain-containing protein -
NML26_RS25025 (NML26_25035) 84995..85276 - 282 WP_000077926.1 hypothetical protein -
NML26_RS25030 (NML26_25040) 85326..85517 - 192 WP_000398480.1 hypothetical protein -
NML26_RS25035 (NML26_25045) 85609..85950 - 342 WP_001151575.1 hypothetical protein -
NML26_RS25040 (NML26_25050) 86323..86715 + 393 WP_000341066.1 hypothetical protein -
NML26_RS25045 (NML26_25055) 87319..87612 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NML26_RS25050 (NML26_25060) 87617..88942 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NML26_RS25055 (NML26_25065) 89003..89209 + 207 WP_000134171.1 hypothetical protein -
NML26_RS25060 (NML26_25070) 89311..89721 + 411 WP_000985911.1 hypothetical protein -
NML26_RS25065 (NML26_25075) 89734..90549 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NML26_RS25070 (NML26_25080) 90803..91228 + 426 WP_001043843.1 hypothetical protein -
NML26_RS25075 (NML26_25085) 91777..92085 + 309 WP_001224687.1 hypothetical protein -
NML26_RS25080 (NML26_25090) 92101..92958 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
NML26_RS25085 (NML26_25095) 93020..93223 + 204 WP_001194555.1 hypothetical protein -
NML26_RS25090 (NML26_25100) 93565..93921 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / floR / aph(6)-Id / tet(A) / sul3 / lnu(F) / ant(3'')-Ia / aac(3)-IId / blaCTX-M-55 / blaTEM-1B / fosA3 / mph(A) / qnrS1 / ARR-3 / dfrA14 - 1..274018 274018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T251530 WP_000088046.1 NZ_CP101211:87617-88942 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT251530 WP_024136327.1 NZ_CP101211:87319-87612 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References