Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4587438..4587935 Replicon chromosome
Accession NZ_CP101197
Organism Escherichia coli strain EC21Z-151

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NML27_RS22035 Protein ID WP_001173343.1
Coordinates 4587438..4587611 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NML27_RS22040 Protein ID WP_000398619.1
Coordinates 4587639..4587935 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML27_RS22025 (4584108) 4584108..4585565 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NML27_RS22030 (4585802) 4585802..4587319 + 1518 WP_001550472.1 lysine--tRNA ligase -
NML27_RS22035 (4587438) 4587438..4587611 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NML27_RS22040 (4587639) 4587639..4587935 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NML27_RS22045 (4588164) 4588164..4588436 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NML27_RS22050 (4588448) 4588448..4588678 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NML27_RS22055 (4588858) 4588858..4590489 + 1632 WP_001216477.1 sensor histidine kinase -
NML27_RS22060 (4590486) 4590486..4591205 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4563526..4587935 24409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251491 WP_001173343.1 NZ_CP101197:c4587611-4587438 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251491 WP_000398619.1 NZ_CP101197:c4587935-4587639 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References