Detailed information of TA system TA251486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3941220..3942909
• Replicon: chromosome
• Accession: NZ_CP101197
• Organism: Escherichia coli strain EC21Z-151

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: NML27_RS18935
• Protein ID: WP_000365809.1
• Coordinates: 3941220..3942140 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NML27_RS18940
• Protein ID: WP_001550661.1
• Coordinates: 3942127..3942909 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NML27_RS18920 (3937310)	3937310..3938437	-	1128	WP_001550663.1	MFS transporter	-
NML27_RS18925 (3938579)	3938579..3939637	-	1059	WP_122987197.1	alpha/beta hydrolase	-
NML27_RS18930 (3939902)	3939902..3940786	+	885	WP_001119037.1	LysR family transcriptional regulator	-
NML27_RS18935 (3941220)	3941220..3942140	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NML27_RS18940 (3942127)	3942127..3942909	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
NML27_RS18945 (3943225)	3943225..3946434	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
NML27_RS18950 (3946781)	3946781..3947251	+	471	WP_000201185.1	hypothetical protein	-
NML27_RS18955 (3947248)	3947248..3947350	+	103	Protein_3709	helix-turn-helix domain-containing protein	-
NML27_RS18960 (3947442)	3947442..3947600	+	159	Protein_3710	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T251486 WP_000365809.1 NZ_CP101197:c3942140-3941220 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT251486 WP_001550661.1 NZ_CP101197:c3942909-3942127 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0