Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3076002..3076786 Replicon chromosome
Accession NZ_CP101197
Organism Escherichia coli strain EC21Z-151

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NML27_RS14990 Protein ID WP_000613626.1
Coordinates 3076292..3076786 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag NML27_RS14985 Protein ID WP_001110447.1
Coordinates 3076002..3076295 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML27_RS14975 (3071152) 3071152..3072111 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
NML27_RS14980 (3072684) 3072684..3075869 + 3186 WP_001550951.1 ribonuclease E -
NML27_RS14985 (3076002) 3076002..3076295 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
NML27_RS14990 (3076292) 3076292..3076786 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NML27_RS14995 (3076881) 3076881..3077834 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
NML27_RS15000 (3077846) 3077846..3079489 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
NML27_RS15005 (3079555) 3079555..3080496 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NML27_RS15010 (3080496) 3080496..3081593 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T251483 WP_000613626.1 NZ_CP101197:3076292-3076786 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT251483 WP_001110447.1 NZ_CP101197:3076002-3076295 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References