Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1637213..1637769 Replicon chromosome
Accession NZ_CP100764
Organism Serratia plymuthica strain SASK2000

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7U4DW07
Locus tag NLX81_RS07855 Protein ID WP_013812173.1
Coordinates 1637452..1637769 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A7U3Z0I2
Locus tag NLX81_RS07850 Protein ID WP_013812172.1
Coordinates 1637213..1637449 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLX81_RS07835 (NLX81_07835) 1633639..1635177 + 1539 WP_013812169.1 glutathione ABC transporter substrate-binding protein GsiB -
NLX81_RS07840 (NLX81_07840) 1635230..1636150 + 921 WP_254754944.1 glutathione ABC transporter permease GsiC -
NLX81_RS07845 (NLX81_07845) 1636160..1637068 + 909 WP_013812171.1 glutathione ABC transporter permease GsiD -
NLX81_RS07850 (NLX81_07850) 1637213..1637449 + 237 WP_013812172.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
NLX81_RS07855 (NLX81_07855) 1637452..1637769 + 318 WP_013812173.1 CcdB family protein Toxin
NLX81_RS07860 (NLX81_07860) 1637803..1638645 - 843 WP_004942338.1 S-formylglutathione hydrolase -
NLX81_RS07865 (NLX81_07865) 1638660..1639787 - 1128 WP_004942340.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
NLX81_RS07870 (NLX81_07870) 1639816..1640733 - 918 WP_013812174.1 LysR family transcriptional regulator -
NLX81_RS07875 (NLX81_07875) 1640841..1641998 + 1158 WP_013812175.1 YbfB/YjiJ family MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11972.92 Da        Isoelectric Point: 5.8267

>T251380 WP_013812173.1 NZ_CP100764:1637452-1637769 [Serratia plymuthica]
MQFTVYANTGKSAVYPLLLDVTNDIIEQLNRRVMIPLLPIEKYPASTRPERLNPLVRLVDDNEYAVMTHEMASIPVRILG
AEFCDASQYRTKVKAAIDFLFDGIR

Download         Length: 318 bp

>T251380 NZ_CP100764:1637452-1637769 [Serratia plymuthica]
ATGCAATTTACGGTATACGCGAATACGGGGAAAAGCGCAGTCTATCCTCTGCTGCTTGATGTGACGAACGATATCATCGA
GCAATTAAATCGCCGAGTTATGATCCCGTTATTACCCATTGAAAAGTATCCGGCCAGTACTCGCCCGGAACGCTTGAACC
CTTTGGTCAGGCTGGTGGATGACAACGAGTACGCCGTTATGACTCATGAGATGGCAAGTATTCCCGTTCGTATCCTGGGC
GCTGAATTTTGCGATGCTTCCCAATACCGAACCAAGGTGAAAGCCGCAATAGATTTTCTTTTCGACGGTATCAGATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9184.30 Da        Isoelectric Point: 6.7189

>AT251380 WP_013812172.1 NZ_CP100764:1637213-1637449 [Serratia plymuthica]
MPVKQRTTQSVTMAMERSLLSRAREAGINLSTTLNTALDAELRQHEMRKWKEENREAIDALNRFHEEHGCFSDEYRTF

Download         Length: 237 bp

>AT251380 NZ_CP100764:1637213-1637449 [Serratia plymuthica]
ATGCCAGTAAAACAACGAACGACCCAAAGCGTTACCATGGCGATGGAACGCAGTTTACTGAGCCGTGCACGTGAGGCGGG
CATTAACCTCAGTACTACGCTGAATACTGCTTTGGATGCCGAATTGCGTCAGCATGAGATGAGGAAATGGAAGGAAGAAA
ACCGAGAAGCGATCGATGCTTTAAATCGTTTTCATGAAGAACATGGCTGTTTCAGTGACGAATACAGGACGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4DW07

Antitoxin

Source ID Structure
AlphaFold DB A0A7U3Z0I2

References