Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 524005..524641 Replicon chromosome
Accession NZ_CP100752
Organism Bacillus halotolerans strain MEC_B301

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NLV76_RS02705 Protein ID WP_003156187.1
Coordinates 524291..524641 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NLV76_RS02700 Protein ID WP_003225183.1
Coordinates 524005..524286 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLV76_RS02680 (NLV76_02680) 520362..520961 - 600 WP_254502533.1 rhomboid family intramembrane serine protease -
NLV76_RS02685 (NLV76_02685) 521056..521421 + 366 WP_254502535.1 holo-ACP synthase -
NLV76_RS02690 (NLV76_02690) 521587..522603 + 1017 WP_082687822.1 outer membrane lipoprotein carrier protein LolA -
NLV76_RS02695 (NLV76_02695) 522720..523904 + 1185 WP_254502536.1 alanine racemase -
NLV76_RS02700 (NLV76_02700) 524005..524286 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NLV76_RS02705 (NLV76_02705) 524291..524641 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NLV76_RS02710 (NLV76_02710) 524757..525581 + 825 WP_254502537.1 RsbT co-antagonist protein RsbRA -
NLV76_RS02715 (NLV76_02715) 525586..525951 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NLV76_RS02720 (NLV76_02720) 525955..526356 + 402 WP_254502538.1 serine/threonine-protein kinase RsbT -
NLV76_RS02725 (NLV76_02725) 526368..527375 + 1008 WP_254502539.1 phosphoserine phosphatase RsbU -
NLV76_RS02730 (NLV76_02730) 527436..527765 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
NLV76_RS02735 (NLV76_02735) 527762..528244 + 483 WP_024120302.1 anti-sigma B factor RsbW -
NLV76_RS02740 (NLV76_02740) 528210..528998 + 789 WP_254502540.1 RNA polymerase sigma factor SigB -
NLV76_RS02745 (NLV76_02745) 528998..529597 + 600 WP_044161719.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T251352 WP_003156187.1 NZ_CP100752:524291-524641 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT251352 WP_003225183.1 NZ_CP100752:524005-524286 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References