Detailed information of TA system TA025134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 354747..355533
• Replicon: chromosome
• Accession: NC_015186
• Organism: Acidiphilium multivorum AIU301

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: F0J270
• Locus tag: ACMV_RS01520
• Protein ID: WP_013639296.1
• Coordinates: 355042..355533 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: ACMV_RS01515
• Protein ID: WP_148360949.1
• Coordinates: 354747..355040 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACMV_RS01495	350292..350930	+	639	WP_011941407.1	hypothetical protein	-
ACMV_RS01500	351192..351524	+	333	WP_011941408.1	helix-turn-helix domain-containing protein	-
ACMV_RS01505	351521..353578	+	2058	WP_013639293.1	tyrosine-type recombinase/integrase	-
ACMV_RS01510	353583..354665	+	1083	WP_013639294.1	site-specific integrase	-
ACMV_RS01515	354747..355040	+	294	WP_148360949.1	DUF1778 domain-containing protein	Antitoxin
ACMV_RS01520	355042..355533	+	492	WP_013639296.1	GNAT family N-acetyltransferase	Toxin
ACMV_RS01525	355824..356162	+	339	Protein_306	hypothetical protein	-
ACMV_RS01530	356229..356771	+	543	WP_011941414.1	transposase	-
ACMV_RS19550	356827..358557	-	1731	WP_081479217.1	hypothetical protein	-
ACMV_RS20665	358559..358654	+	96	Protein_309	IS5/IS1182 family transposase	-
ACMV_RS19555	358767..359150	+	384	WP_196796274.1	helix-turn-helix domain-containing protein	-
ACMV_RS01535	359110..360333	-	1224	WP_013639301.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	351264..371803	20539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (17-91)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17293.92 Da
• Isoelectric Point: 10.1522

>T25134 WP_013639296.1 NC_015186:355042-355533 [Acidiphilium multivorum AIU301]
VALTPPEPLAEHHDISGFNCGIASLDDWLRRRARANQAGGATRTFVVTESGRVVAYYAIASGAISPNGTSGRFRRNMPDP
IPVAVLARLAIATSAQGQGLGRALFRDATLRILNAAETLGIRGIVVHAISDQAKAFYLALGFEASPLDPMTLMVTLKDVR
AVL

Nucleotide

• Length: 492 bp

>T25134 NC_015186:355042-355533 [Acidiphilium multivorum AIU301]
GTGGCCCTCACCCCGCCCGAACCGCTCGCCGAGCATCACGACATCAGCGGCTTCAACTGCGGCATCGCCTCACTGGATGA
CTGGCTGAGGCGCCGGGCCCGCGCCAACCAGGCCGGCGGCGCGACCCGTACCTTCGTTGTGACCGAAAGCGGCCGCGTCG
TCGCCTATTACGCCATCGCTTCCGGCGCCATCAGCCCGAACGGCACCTCCGGCCGCTTCCGCCGCAACATGCCCGACCCG
ATCCCCGTCGCCGTCCTCGCCCGCCTCGCAATCGCCACCAGCGCCCAGGGACAAGGCCTCGGCCGCGCCCTCTTCCGCGA
CGCCACCCTCCGCATCCTGAACGCAGCCGAAACGCTGGGGATCAGAGGCATCGTGGTGCATGCCATCTCCGACCAGGCCA
AAGCCTTCTACCTCGCCCTCGGCTTCGAAGCCTCGCCGCTCGATCCGATGACGCTGATGGTCACGCTCAAGGATGTGAGA
GCGGTATTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10744.20 Da
• Isoelectric Point: 10.5728

>AT25134 WP_148360949.1 NC_015186:354747-355040 [Acidiphilium multivorum AIU301]
MNPAKQASAPSSRRDTLNLRIKSDDRGLIDRAAALTGKTKTDFVIDAARRAAEDALLDRTLFLATPDAFDAFRARLDEPP
LPNARLQRALKTTPPWE

Nucleotide

• Length: 294 bp

>AT25134 NC_015186:354747-355040 [Acidiphilium multivorum AIU301]
ATGAATCCCGCCAAGCAAGCCTCTGCGCCATCCTCCCGCCGCGACACGCTCAACCTGCGGATCAAAAGCGACGATCGCGG
GCTGATCGACCGCGCCGCCGCCCTGACCGGCAAGACCAAGACGGACTTCGTGATCGACGCCGCCCGCCGCGCCGCCGAAG
ACGCCCTGCTCGACCGCACGCTGTTTCTCGCAACTCCGGATGCGTTCGACGCCTTCCGCGCGCGCCTCGACGAACCGCCG
CTGCCGAATGCCCGCCTCCAGCGCGCCCTGAAGACAACGCCGCCCTGGGAATGA

Structures

Toxin

• AlphaFold DB: F0J270