Detailed information of TA system TA025133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 373412..374082
• Replicon: plasmid pAspH13-3a
• Accession: NC_015184
• Organism: Agrobacterium sp. H13-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AGROH133_RS25285
• Protein ID: WP_003517201.1
• Coordinates: 373663..374082 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F0LGD9
• Locus tag: AGROH133_RS25280
• Protein ID: WP_003517203.1
• Coordinates: 373412..373666 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGROH133_RS25270	370883..371890	+	1008	WP_013637428.1	PAS domain-containing protein	-
AGROH133_RS25275	372423..373028	-	606	WP_013637430.1	J domain-containing protein	-
AGROH133_RS25280	373412..373666	+	255	WP_003517203.1	plasmid stabilization protein	Antitoxin
AGROH133_RS25285	373663..374082	+	420	WP_003517201.1	type II toxin-antitoxin system VapC family toxin	Toxin
AGROH133_RS25290	374222..375118	-	897	WP_013637432.1	dihydrodipicolinate synthase family protein	-
AGROH133_RS25295	375151..376032	-	882	WP_003517197.1	SMP-30/gluconolactonase/LRE family protein	-
AGROH133_RS25300	376089..376808	-	720	WP_003517195.1	FadR family transcriptional regulator	-
AGROH133_RS25305	377108..379057	+	1950	WP_013637433.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..601551	601551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15036.22 Da
• Isoelectric Point: 5.1532

>T25133 WP_003517201.1 NC_015184:373663-374082 [Agrobacterium fabacearum]
MILLDTNVISEPWKPVPDEAVIAWLDAQAVETLFISAITIAELRFGIAAMPSGRRQTILRDRLEGEVLPHFSGRILSFDL
TTSQFYSELMARARASGKAIGTADGYIAATAAANGLTISTRDTSPFEAAGVKVINPWSR

Nucleotide

• Length: 420 bp

>T25133 NC_015184:373663-374082 [Agrobacterium fabacearum]
ATGATCTTGCTTGACACCAACGTCATATCCGAACCTTGGAAACCGGTTCCGGACGAGGCGGTGATTGCCTGGTTGGACGC
CCAAGCTGTCGAAACGCTGTTCATCTCCGCAATAACGATTGCGGAACTTCGCTTCGGAATTGCTGCTATGCCTTCAGGCA
GACGGCAAACTATCCTCCGCGACCGTCTCGAGGGCGAGGTGCTGCCACACTTTTCCGGCCGCATCCTGTCCTTCGATCTG
ACTACTTCTCAGTTCTACTCTGAACTGATGGCACGCGCTCGAGCGTCTGGGAAAGCAATCGGCACGGCCGATGGCTACAT
TGCAGCCACAGCGGCAGCAAATGGCCTCACTATCTCAACTCGCGATACAAGCCCTTTCGAGGCTGCCGGGGTGAAAGTGA
TCAACCCCTGGTCCAGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9253.53 Da
• Isoelectric Point: 8.9520

>AT25133 WP_003517203.1 NC_015184:373412-373666 [Agrobacterium fabacearum]
MPAVTIRNLSEETHRALRVRAAHHGRSTEAEIRAIIEAAVRPPERIQLGSLLAAIGRDAELSNSDVEALQKSRDKTPAKP
MTFE

Nucleotide

• Length: 255 bp

>AT25133 NC_015184:373412-373666 [Agrobacterium fabacearum]
ATGCCGGCAGTCACCATTCGTAACCTTTCTGAAGAAACACATCGCGCTCTGAGGGTGCGAGCGGCTCATCATGGCCGGAG
CACGGAGGCCGAGATTCGTGCCATTATTGAAGCGGCTGTTCGTCCTCCGGAACGTATTCAGCTCGGTTCCTTGCTAGCGG
CCATTGGTCGCGACGCCGAGCTTTCCAACAGCGACGTTGAAGCTCTTCAGAAAAGTCGCGACAAGACACCTGCTAAGCCG
ATGACCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: F0LGD9