Detailed information of TA system TA025132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 232185..232765
• Replicon: plasmid pAspH13-3a
• Accession: NC_015184
• Organism: Agrobacterium sp. H13-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F0LFX9
• Locus tag: AGROH133_RS24585
• Protein ID: WP_013637281.1
• Coordinates: 232382..232765 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F0LFX8
• Locus tag: AGROH133_RS24580
• Protein ID: WP_013637280.1
• Coordinates: 232185..232385 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGROH133_RS24560	227714..228724	+	1011	WP_013637275.1	alpha/beta hydrolase	-
AGROH133_RS24565	228746..229777	+	1032	WP_013637276.1	ankyrin repeat domain-containing protein	-
AGROH133_RS24570	229966..230907	-	942	WP_013637277.1	NmrA family NAD(P)-binding protein	-
AGROH133_RS24575	230982..231785	+	804	WP_013637278.1	helix-turn-helix transcriptional regulator	-
AGROH133_RS24580	232185..232385	+	201	WP_013637280.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AGROH133_RS24585	232382..232765	+	384	WP_013637281.1	type II toxin-antitoxin system VapC family toxin	Toxin
AGROH133_RS24590	232980..233677	-	698	Protein_251	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
AGROH133_RS24595	233689..233952	-	264	WP_013637284.1	hypothetical protein	-
AGROH133_RS24600	234142..234858	-	717	Protein_253	D-alanine--D-alanine ligase	-
AGROH133_RS24605	235157..236053	+	897	WP_013637286.1	transglutaminase family protein	-
AGROH133_RS24610	236156..236698	+	543	Protein_255	IS6 family transposase	-
AGROH133_RS27880	236668..236811	-	144	WP_162686747.1	hypothetical protein	-
AGROH133_RS26975	236808..237481	-	674	Protein_257	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..601551	601551
	-	flank	IS/Tn	-	-	236156..236713	557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14252.35 Da
• Isoelectric Point: 6.8437

>T25132 WP_013637281.1 NC_015184:232382-232765 [Agrobacterium fabacearum]
VILVDTSIWINHFRYDDSELRKIINDDQLLCHPFVVGELALGSLRERAAVLEFLAAQREALIATHAEVMTIIDRHSIFSM
GIGYTDAHLLTSTLLDRRSSLWTRDKRLVAAAQKVGAALYTHAQASH

Nucleotide

• Length: 384 bp

>T25132 NC_015184:232382-232765 [Agrobacterium fabacearum]
GTGATCCTGGTAGACACCTCGATCTGGATAAATCATTTTCGATACGATGACTCAGAACTACGAAAAATCATCAATGATGA
CCAGTTGCTCTGCCACCCATTCGTTGTAGGCGAATTGGCGCTCGGCAGCTTAAGAGAGCGGGCTGCAGTTTTAGAATTCC
TAGCAGCTCAACGCGAGGCGCTAATCGCGACCCATGCAGAAGTAATGACGATCATTGATCGTCATTCGATATTCAGCATG
GGGATTGGTTACACAGATGCGCATTTACTTACTTCAACGTTGCTCGACCGGCGATCAAGCTTGTGGACAAGAGATAAGCG
CCTAGTGGCCGCAGCACAAAAAGTTGGTGCGGCACTTTACACGCACGCGCAAGCCTCTCATTAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7197.28 Da
• Isoelectric Point: 10.7407

>AT25132 WP_013637280.1 NC_015184:232185-232385 [Agrobacterium fabacearum]
MRSTINIDDNLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGSMPEAEASPRRRSDVAK

Nucleotide

• Length: 201 bp

>AT25132 NC_015184:232185-232385 [Agrobacterium fabacearum]
ATGCGATCAACCATAAATATCGACGACAATCTTATGGAACGGGCCAAGTCCCTCACGGGCACCAAGGAAACAGCAGCTCT
CGTGCGGCAAGCGCTGGAGACACTCGTGCGTGTGGAATCCGGCAAACGGCTGATCGCGCTTGGCGGCTCTATGCCTGAGG
CTGAAGCGTCGCCTCGCCGACGGAGCGACGTGGCAAAGTGA

Structures

Toxin

• AlphaFold DB: F0LFX9

Antitoxin

• AlphaFold DB: F0LFX8