Detailed information of TA system TA025131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 629409..629889
• Replicon: chromosome
• Accession: NC_015183
• Organism: Agrobacterium sp. H13-3

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: F0L6V4
• Locus tag: AGROH133_RS03040
• Protein ID: WP_013635547.1
• Coordinates: 629409..629678 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F0L6V5
• Locus tag: AGROH133_RS03045
• Protein ID: WP_013635548.1
• Coordinates: 629662..629889 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGROH133_RS03010	624447..625220	-	774	WP_004432233.1	L,D-transpeptidase	-
AGROH133_RS03015	625458..626087	+	630	WP_013635543.1	DNA-3-methyladenine glycosylase I	-
AGROH133_RS03020	626084..626527	+	444	WP_013635544.1	hypothetical protein	-
AGROH133_RS03025	626543..627586	-	1044	WP_019564245.1	YeiH family protein	-
AGROH133_RS03030	627669..628586	+	918	WP_004432220.1	LysR family transcriptional regulator	-
AGROH133_RS03035	628638..629402	-	765	WP_013635546.1	hypothetical protein	-
AGROH133_RS03040	629409..629678	-	270	WP_013635547.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AGROH133_RS03045	629662..629889	-	228	WP_013635548.1	ribbon-helix-helix protein, CopG family	Antitoxin
AGROH133_RS03050	630046..631569	+	1524	WP_013635549.1	histidine--tRNA ligase	-
AGROH133_RS03055	631574..631942	+	369	WP_041694516.1	VOC family protein	-
AGROH133_RS03060	631959..633086	+	1128	WP_004432209.1	ATP phosphoribosyltransferase regulatory subunit	-
AGROH133_RS03065	633083..633775	+	693	WP_004432208.1	ATP phosphoribosyltransferase	-
AGROH133_RS03070	633839..634285	-	447	WP_013635551.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10586.19 Da
• Isoelectric Point: 9.5682

>T25131 WP_013635547.1 NC_015183:c629678-629409 [Agrobacterium fabacearum]
VIWTIEYDVLVQKEMRKIDSQTRQRIRAFLQERVAALDNPRQTGAALQGSELGSFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T25131 NC_015183:c629678-629409 [Agrobacterium fabacearum]
GTGATCTGGACAATTGAATACGATGTTCTTGTCCAGAAAGAGATGCGGAAAATCGATTCGCAGACGCGTCAGCGTATCCG
CGCTTTCCTTCAAGAGAGAGTAGCGGCGCTCGACAATCCCCGCCAGACAGGTGCCGCCCTGCAAGGCTCAGAACTCGGCA
GCTTCTGGCGTTACCGGGTAGGCGATTACCGTATCATCTGCGATATACAGGACCACAAGCTTGTCGTGCTGGTGGTGGAA
ATTGGCCATCGCCGCGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8909.92 Da
• Isoelectric Point: 4.7254

>AT25131 WP_013635548.1 NC_015183:c629889-629662 [Agrobacterium fabacearum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKVVSAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT25131 NC_015183:c629889-629662 [Agrobacterium fabacearum]
ATGAGCAAACAGACTGCCATCCGCCTGCCGGACGAGACCTATGAACGGCTGAAAGCGCTTTCCGAGCGCACGGGCCGTAC
GTCCGCTTACTATATCCGCGAGGCGATTGAGAAACATATTGAGGATATGGAAGATCTCTATTTAGCCGAAGAGGCGACGC
GACGCATCCAGCGCGGTGAATCAAAGGTGGTAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Toxin

• AlphaFold DB: F0L6V4

Antitoxin

• AlphaFold DB: F0L6V5