Detailed information of TA system TA025129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 16586..17066
• Replicon: plasmid pACMV3
• Accession: NC_015179
• Organism: Acidiphilium multivorum AIU301

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: F0J7U9
• Locus tag: ACMV_RS18765
• Protein ID: WP_013635096.1
• Coordinates: 16586..16858 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F0J7V0
• Locus tag: ACMV_RS18770
• Protein ID: WP_013635097.1
• Coordinates: 16842..17066 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACMV_RS21045	12234..12380	-	147	WP_154653566.1	hypothetical protein	-
ACMV_RS18750	12605..13597	-	993	WP_013635093.1	ABC transporter permease	-
ACMV_RS18755	13598..14641	-	1044	WP_013635094.1	ABC transporter permease	-
ACMV_RS18760	14662..16317	-	1656	WP_041665874.1	ABC transporter substrate-binding protein	-
ACMV_RS18765	16586..16858	-	273	WP_013635096.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACMV_RS18770	16842..17066	-	225	WP_013635097.1	TraY domain-containing protein	Antitoxin
ACMV_RS18775	17335..17649	-	315	Protein_20	IS1634 family transposase	-
ACMV_RS18780	17646..18044	-	399	WP_148360932.1	type II toxin-antitoxin system VapC family toxin	-
ACMV_RS18785	18059..18319	-	261	WP_013635100.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ACMV_RS18790	18410..19327	-	918	WP_013635101.1	hypothetical protein	-
ACMV_RS18795	19426..20133	-	708	WP_013635102.1	mobilization protein	-
ACMV_RS18800	20130..20618	-	489	WP_013635103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54248	54248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Protein of unknown function DUF6290 (3-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10430.12 Da
• Isoelectric Point: 10.7225

>T25129 WP_013635096.1 NC_015179:c16858-16586 [Acidiphilium multivorum AIU301]
MAWKIELDPAADRELDKIDKQTARRILAFLHDRVAALDNPRSIGEALKGSKLGDFWKYRVGNWRIIASIEDGALRILIVR
IGNRREVYKR

Nucleotide

• Length: 273 bp

>T25129 NC_015179:c16858-16586 [Acidiphilium multivorum AIU301]
ATGGCCTGGAAGATTGAGCTCGATCCTGCTGCCGATCGTGAACTTGATAAAATCGACAAGCAAACCGCTCGCCGCATTCT
CGCCTTTCTCCATGACCGGGTTGCTGCGCTAGACAATCCACGCAGCATTGGCGAAGCCCTTAAAGGTTCCAAGCTGGGGG
ATTTCTGGAAGTACCGCGTAGGCAACTGGCGAATTATCGCCAGCATCGAGGACGGCGCTTTGCGTATCCTCATTGTCAGA
ATCGGCAACCGACGCGAAGTTTATAAGCGCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8456.72 Da
• Isoelectric Point: 4.6286

>AT25129 WP_013635097.1 NC_015179:c17066-16842 [Acidiphilium multivorum AIU301]
VLAVRLPPEIESRLDALAQATGRAKSFYVREAIMEHLDDLEDLYLAEQRLIDLRAGKTKTVPLEDVMKRYGLED

Nucleotide

• Length: 225 bp

>AT25129 NC_015179:c17066-16842 [Acidiphilium multivorum AIU301]
GTGCTGGCTGTCCGCCTTCCCCCTGAAATCGAGAGCCGCCTTGATGCGTTGGCCCAGGCTACAGGCCGAGCCAAGAGCTT
TTACGTCCGTGAAGCGATCATGGAGCATTTAGACGACCTCGAAGATTTATATCTTGCGGAACAACGCCTGATTGACTTGC
GGGCTGGCAAAACCAAGACCGTTCCGCTCGAAGATGTAATGAAGCGGTATGGCCTGGAAGATTGA

Structures

Toxin

• AlphaFold DB: F0J7U9

Antitoxin

• AlphaFold DB: F0J7V0