Detailed information of TA system TA025128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 227202..227733
• Replicon: plasmid pACMV1
• Accession: NC_015178
• Organism: Acidiphilium multivorum AIU301

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F0J7G2
• Locus tag: ACMV_RS18180
• Protein ID: WP_013635040.1
• Coordinates: 227202..227483 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: F0J7G3
• Locus tag: ACMV_RS18185
• Protein ID: WP_013635041.1
• Coordinates: 227470..227733 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACMV_RS21000	222240..222688	-	449	Protein_223	cell filamentation protein	-
ACMV_RS18150	222712..222921	-	210	WP_013635038.1	hypothetical protein	-
ACMV_RS19410	223074..223493	-	420	Protein_225	hypothetical protein	-
ACMV_RS21005	223497..223733	-	237	Protein_226	hypothetical protein	-
ACMV_RS18165	223812..225023	+	1212	WP_011930445.1	tyrosine-type recombinase/integrase	-
ACMV_RS18170	225020..225961	+	942	WP_011930444.1	tyrosine-type recombinase/integrase	-
ACMV_RS18175	225958..226950	+	993	WP_011930443.1	site-specific integrase	-
ACMV_RS21010	227043..227189	-	147	WP_154653557.1	hypothetical protein	-
ACMV_RS18180	227202..227483	-	282	WP_013635040.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ACMV_RS18185	227470..227733	-	264	WP_013635041.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ACMV_RS18190	228272..229159	-	888	WP_013635042.1	LysR family transcriptional regulator	-
ACMV_RS18195	229289..230968	-	1680	WP_013635043.1	IS1634 family transposase	-
ACMV_RS18200	231323..232114	+	792	WP_048858261.1	enoyl-CoA hydratase/isomerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..271573	271573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10760.22 Da
• Isoelectric Point: 8.1371

>T25128 WP_013635040.1 NC_015178:c227483-227202 [Acidiphilium multivorum AIU301]
MRTPDYTGQFKRDYKREKKGRHRATLDADLHAVLSALLADLPLEPRHRDHALSGDWKDHRDCHVRPDLVLIYQLPDAGTL
RLVRLGSHSELDL

Nucleotide

• Length: 282 bp

>T25128 NC_015178:c227483-227202 [Acidiphilium multivorum AIU301]
ATGCGGACGCCTGACTATACCGGCCAGTTCAAGCGCGACTACAAACGCGAGAAGAAGGGTCGGCATCGCGCCACGCTCGA
CGCGGACCTGCACGCCGTCCTGTCCGCGTTGCTGGCCGATCTGCCGCTGGAACCACGCCATCGCGATCACGCGCTGAGCG
GCGATTGGAAAGACCATCGGGATTGCCACGTGAGGCCCGACCTGGTGCTGATCTACCAGCTTCCCGACGCTGGCACGCTT
CGCCTTGTGCGCCTTGGCTCCCATTCTGAACTCGACCTGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9201.63 Da
• Isoelectric Point: 4.9670

>AT25128 WP_013635041.1 NC_015178:c227733-227470 [Acidiphilium multivorum AIU301]
MAANQLVQTRIDGAIKEEAATVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIQAMKDARAGRVTKAADLDTLF
HDLNADA

Nucleotide

• Length: 264 bp

>AT25128 NC_015178:c227733-227470 [Acidiphilium multivorum AIU301]
ATGGCTGCCAACCAGCTAGTGCAAACTCGCATCGATGGCGCGATCAAAGAGGAGGCGGCTACGGTGTTGGCCGCCATGGG
TTTGACCGTCTCCGATGCGGTCAGGCTGCTGCTCACGAAGGTGGCGCAGGACAAGGCACTGCCTTTCGAGCCGCTGATCC
CGAACGCGACGACGATTCAGGCGATGAAGGACGCACGAGCCGGCCGCGTCACGAAGGCGGCCGATCTCGATACGCTGTTC
CATGATCTCAATGCGGACGCCTGA

Structures

Toxin

• AlphaFold DB: F0J7G2

Antitoxin

• AlphaFold DB: F0J7G3