Detailed information of TA system TA025125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2047..2666
• Replicon: plasmid pDEIPR04
• Accession: NC_015163
• Organism: Deinococcus proteolyticus MRP

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DEIPR_RS13300
• Protein ID: WP_041223179.1
• Coordinates: 2334..2666 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: F0RR36
• Locus tag: DEIPR_RS13295
• Protein ID: WP_013615990.1
• Coordinates: 2047..2322 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEIPR_RS13285	335..1540	+	1206	WP_174260429.1	transcriptional regulator	-
DEIPR_RS13290	1525..1953	-	429	WP_013615989.1	hypothetical protein	-
DEIPR_RS13295	2047..2322	+	276	WP_013615990.1	antitoxin	Antitoxin
DEIPR_RS13300	2334..2666	+	333	WP_041223179.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DEIPR_RS13305	2659..3036	-	378	WP_013615992.1	hypothetical protein	-
DEIPR_RS13310	3040..3654	-	615	WP_013615993.1	C39 family peptidase	-
DEIPR_RS13315	3758..4204	+	447	WP_013615994.1	DUF2384 domain-containing protein	-
DEIPR_RS13320	4232..4738	+	507	WP_013615995.1	RES family NAD+ phosphorylase	-
DEIPR_RS13325	4714..5091	-	378	WP_148232020.1	hypothetical protein	-
DEIPR_RS13330	5171..5569	-	399	WP_013615997.1	hypothetical protein	-
DEIPR_RS13335	5566..6129	-	564	WP_013615998.1	hypothetical protein	-
DEIPR_RS13340	6747..7321	+	575	Protein_11	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97188	97188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

• Antitoxin PemI-like (6-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11802.60 Da
• Isoelectric Point: 8.0235

>T25125 WP_041223179.1 NC_015163:2334-2666 [Deinococcus proteolyticus MRP]
IRRGEIYLVDLNPTAGHEQRGKRPVLVVSPNAFNALTGTPIVLPVTSGGQFARMAGFAVSLDEAGTQTTGIVRCDQPRAL
DFSARGARLLEMVPRDVVDEVLARLAVLFS

Nucleotide

• Length: 333 bp

>T25125 NC_015163:2334-2666 [Deinococcus proteolyticus MRP]
ATCAGACGGGGCGAGATTTACCTGGTGGATTTGAATCCTACGGCCGGACATGAGCAGCGTGGCAAGCGTCCAGTCCTGGT
GGTGTCGCCGAATGCCTTCAACGCCCTGACTGGTACGCCCATCGTGCTGCCAGTGACCAGCGGAGGACAGTTCGCACGGA
TGGCCGGGTTTGCGGTGTCCCTGGACGAAGCTGGAACCCAGACCACGGGCATCGTGCGCTGTGATCAGCCCAGGGCACTT
GATTTCAGCGCGCGGGGAGCCCGCCTTCTTGAGATGGTGCCCCGGGATGTGGTGGATGAGGTGCTGGCGCGTCTGGCGGT
ACTGTTCAGCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9690.04 Da
• Isoelectric Point: 4.2542

>AT25125 WP_013615990.1 NC_015163:2047-2322 [Deinococcus proteolyticus MRP]
VHKTYLRKVGGSVMLSIPPALLDVLELTAGTAVGLNVEGGQLTVQPAPKPRYSLEELLAQCDLEAQCDPAGAQDAEEQHW
LSDAPVGRELL

Nucleotide

• Length: 276 bp

>AT25125 NC_015163:2047-2322 [Deinococcus proteolyticus MRP]
GTGCATAAGACATACCTGAGAAAAGTCGGCGGTTCGGTGATGTTGAGTATTCCCCCTGCATTGCTGGACGTTCTGGAATT
GACGGCAGGCACCGCTGTGGGCCTGAACGTCGAAGGGGGACAGTTGACGGTTCAGCCAGCCCCAAAACCGCGTTACTCCC
TGGAAGAACTGCTGGCGCAGTGTGATTTAGAAGCGCAGTGTGATCCAGCAGGAGCGCAGGACGCAGAAGAACAGCATTGG
CTGAGTGACGCGCCGGTGGGGCGTGAGCTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: F0RR36