Detailed information of TA system TA025124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2644850..2645381
• Replicon: chromosome
• Accession: NC_015152
• Organism: Sphaerochaeta globosa str. Buddy

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F0RRS1
• Locus tag: SPIBUDDY_RS12345
• Protein ID: WP_013608098.1
• Coordinates: 2645106..2645381 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F0RRS0
• Locus tag: SPIBUDDY_RS12340
• Protein ID: WP_013608097.1
• Coordinates: 2644850..2645113 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPIBUDDY_RS12315	2640595..2641350	-	756	WP_013608093.1	DeoR/GlpR family DNA-binding transcription regulator	-
SPIBUDDY_RS16220	2641593..2641736	+	144	WP_155816109.1	hypothetical protein	-
SPIBUDDY_RS12320	2642234..2642914	-	681	WP_013608094.1	MBL fold metallo-hydrolase	-
SPIBUDDY_RS16225	2643527..2643769	+	243	WP_155816111.1	hypothetical protein	-
SPIBUDDY_RS12330	2643766..2644014	-	249	WP_081454655.1	VF530 family protein	-
SPIBUDDY_RS12335	2644011..2644211	-	201	WP_013608096.1	YwbE family protein	-
SPIBUDDY_RS12340	2644850..2645113	+	264	WP_013608097.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SPIBUDDY_RS12345	2645106..2645381	+	276	WP_013608098.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SPIBUDDY_RS12350	2646066..2647052	+	987	WP_013608100.1	substrate-binding domain-containing protein	-
SPIBUDDY_RS12355	2647180..2648673	+	1494	WP_172634217.1	ATP-binding cassette domain-containing protein	-
SPIBUDDY_RS12360	2648661..2649755	+	1095	WP_013608102.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-85)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10763.42 Da
• Isoelectric Point: 8.9249

>T25124 WP_013608098.1 NC_015152:2645106-2645381 [Sphaerochaeta globosa str. Buddy]
MFDLYFTNQFKKDYRKVSKSGEITLLDEVIDLLRQGLSLPEKFRDHALIDSKKFKSQRECHIKPDWLLVYSVNNEKLVLT
LTRTGSHSELF

Nucleotide

• Length: 276 bp

>T25124 NC_015152:2645106-2645381 [Sphaerochaeta globosa str. Buddy]
ATGTTTGATCTCTATTTCACCAACCAATTCAAGAAAGATTATCGAAAAGTGTCCAAATCCGGGGAGATAACCTTGCTTGA
TGAGGTAATTGACCTGCTGCGGCAAGGACTATCTCTGCCAGAGAAATTTCGCGATCATGCATTGATCGACTCCAAGAAAT
TCAAGAGTCAAAGGGAATGCCACATCAAGCCCGATTGGCTTTTAGTTTACTCTGTGAACAATGAAAAACTTGTCCTTACC
TTGACAAGAACGGGAAGTCATAGCGAATTGTTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9466.96 Da
• Isoelectric Point: 6.2825

>AT25124 WP_013608097.1 NC_015152:2644850-2645113 [Sphaerochaeta globosa str. Buddy]
MSGTITVRVNEKIKAEAQALYESIGLDMSTAINIFLLKSIDMNGIPFNVSKSFPNKETLAAMDDAENGKNLSGPYKSMKE
LKVALNV

Nucleotide

• Length: 264 bp

>AT25124 NC_015152:2644850-2645113 [Sphaerochaeta globosa str. Buddy]
ATGTCTGGAACCATTACCGTTCGAGTGAACGAGAAGATAAAAGCAGAGGCTCAAGCACTTTATGAAAGCATTGGATTGGA
TATGTCCACTGCAATCAACATTTTCCTTTTAAAATCGATTGACATGAATGGAATTCCCTTCAATGTCTCCAAGAGCTTTC
CCAATAAAGAGACTCTCGCAGCAATGGATGATGCAGAGAACGGAAAGAATCTTTCCGGACCATACAAATCGATGAAAGAG
CTAAAAGTTGCCTTGAATGTTTGA

Structures

Toxin

• AlphaFold DB: F0RRS1

Antitoxin

• AlphaFold DB: F0RRS0