Detailed information of TA system TA251220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1014722..1016345
• Replicon: chromosome
• Accession: NZ_CP100724
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain R17.1476

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: NL714_RS04910
• Protein ID: WP_000088046.1
• Coordinates: 1015020..1016345 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: NL714_RS04905
• Protein ID: WP_024136327.1
• Coordinates: 1014722..1015015 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NL714_RS04870 (1010477)	1010477..1010734	-	258	Protein_954	hypothetical protein	-
NL714_RS04875 (1010789)	1010789..1011493	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
NL714_RS04880 (1011557)	1011557..1011835	+	279	Protein_956	VWA domain-containing protein	-
NL714_RS04885 (1012398)	1012398..1012679	-	282	WP_254520351.1	hypothetical protein	-
NL714_RS04890 (1012729)	1012729..1012920	-	192	WP_000398480.1	hypothetical protein	-
NL714_RS04895 (1013012)	1013012..1013353	-	342	WP_001151575.1	hypothetical protein	-
NL714_RS04900 (1013726)	1013726..1014118	+	393	WP_000341066.1	hypothetical protein	-
NL714_RS04905 (1014722)	1014722..1015015	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
NL714_RS04910 (1015020)	1015020..1016345	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
NL714_RS04915 (1016406)	1016406..1016612	+	207	WP_000134171.1	hypothetical protein	-
NL714_RS04920 (1016714)	1016714..1017124	+	411	WP_000985911.1	hypothetical protein	-
NL714_RS04925 (1017137)	1017137..1017952	+	816	WP_053276223.1	HNH endonuclease signature motif containing protein	-
NL714_RS04930 (1018206)	1018206..1018631	+	426	WP_001043843.1	hypothetical protein	-
NL714_RS04935 (1019180)	1019180..1019488	+	309	WP_001224687.1	hypothetical protein	-
NL714_RS04940 (1019504)	1019504..1020361	+	858	WP_000784387.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	invH	1016406..1030409	14003
	-	inside	Prophage	-	invH / invF / invG	1010344..1033200	22856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T251220 WP_000088046.1 NZ_CP100724:1015020-1016345 [Salmonella enterica subsp. enterica serovar Enteritidis]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT251220 WP_024136327.1 NZ_CP100724:1014722-1015015 [Salmonella enterica subsp. enterica serovar Enteritidis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1