Detailed information of TA system TA025122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 756710..757337
• Replicon: chromosome
• Accession: NC_015151
• Organism: Vulcanisaeta moutnovskia 768-28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F0QWE9
• Locus tag: VMUT_RS04060
• Protein ID: WP_013604159.1
• Coordinates: 756710..757117 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F0QWF0
• Locus tag: VMUT_RS04065
• Protein ID: WP_013604160.1
• Coordinates: 757098..757337 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VMUT_RS04030 (VMUT_0780)	751828..752529	-	702	WP_013604153.1	ribose-5-phosphate isomerase RpiA	-
VMUT_RS04035 (VMUT_0781)	752562..753533	-	972	WP_048056858.1	dihydropteroate synthase	-
VMUT_RS04040 (VMUT_0782)	753583..753978	+	396	WP_013604155.1	hypothetical protein	-
VMUT_RS04045 (VMUT_0783)	754001..754999	-	999	WP_013604156.1	sodium:calcium antiporter	-
VMUT_RS04050 (VMUT_0784)	755067..755696	+	630	WP_013604157.1	superoxide dismutase	-
VMUT_RS04055 (VMUT_0785)	755768..756664	-	897	WP_013604158.1	arginase family protein	-
VMUT_RS04060 (VMUT_0786)	756710..757117	-	408	WP_013604159.1	PIN domain-containing protein	Toxin
VMUT_RS04065 (VMUT_0787)	757098..757337	-	240	WP_013604160.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
VMUT_RS04070 (VMUT_0788)	757529..759064	+	1536	WP_237699714.1	putative sulfate exporter family transporter	-
VMUT_RS04075 (VMUT_0789)	759124..760146	+	1023	WP_013604162.1	radical SAM protein	-
VMUT_RS12995	760383..760967	+	585	WP_202795184.1	hypothetical protein	-
VMUT_RS13000	761217..761414	+	198	WP_202795185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (5-48)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15611.25 Da
• Isoelectric Point: 6.9790

>T25122 WP_013604159.1 NC_015151:c757117-756710 [Vulcanisaeta moutnovskia 768-28]
MSEAVIDTNALIFYIVEDSREHTRAVRVLDSLDRWFLHHIVIYELVWFFRSVNVPRDHAMDILGSLINHRKVVLVCDNDI
IKWVLRMMQSTGWGLGGFNDLVVLGTAYALGKPLFTFDEELRIRAKKVGVRVLEV

Nucleotide

• Length: 408 bp

>T25122 NC_015151:c757117-756710 [Vulcanisaeta moutnovskia 768-28]
ATGTCGGAGGCAGTGATTGATACTAATGCGTTGATATTTTACATAGTTGAGGATTCTAGGGAACATACCAGGGCAGTCAG
GGTTTTAGATAGTTTGGACAGATGGTTCTTACATCACATTGTTATTTATGAGCTGGTCTGGTTCTTTAGGTCTGTCAATG
TGCCGAGGGATCACGCTATGGACATTTTAGGTAGTTTAATTAACCATAGGAAGGTGGTTTTAGTATGCGATAATGATATT
ATTAAGTGGGTGTTACGTATGATGCAGAGTACTGGTTGGGGACTTGGTGGTTTTAACGACTTAGTGGTATTAGGAACTGC
ATATGCGTTGGGTAAGCCGTTGTTCACGTTTGATGAGGAGCTCAGGATAAGGGCTAAGAAGGTTGGTGTTAGAGTTCTTG
AGGTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8871.40 Da
• Isoelectric Point: 5.0941

>AT25122 WP_013604160.1 NC_015151:c757337-757098 [Vulcanisaeta moutnovskia 768-28]
VKILVKVTRNYQVTIPASVRERLGIKVGDLLSVEVDDDRIILRKVIQEIPMIRLGRELTISDIDRLIEEGLMSNVGGSD

Nucleotide

• Length: 240 bp

>AT25122 NC_015151:c757337-757098 [Vulcanisaeta moutnovskia 768-28]
GTGAAAATCCTGGTTAAGGTTACTAGAAATTACCAGGTTACGATACCCGCAAGCGTTAGGGAGAGGCTTGGGATTAAGGT
TGGTGATTTATTGAGTGTCGAGGTTGATGATGATCGTATTATCCTTAGGAAGGTAATTCAGGAAATACCAATGATTAGGT
TGGGTAGGGAGTTAACGATCAGTGATATTGATAGGCTCATTGAGGAGGGTTTGATGAGTAATGTCGGAGGCAGTGATTGA

Structures

Toxin

• AlphaFold DB: F0QWE9

Antitoxin

• AlphaFold DB: F0QWF0