Detailed information of TA system TA251193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 191997..193620
• Replicon: plasmid pR17.5474_294k
• Accession: NZ_CP100716
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain R17.5474

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: NL730_RS25440
• Protein ID: WP_000088046.1
• Coordinates: 191997..193322 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: NL730_RS25445
• Protein ID: WP_024136327.1
• Coordinates: 193327..193620 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NL730_RS25405 (NL730_25405)	187627..187953	+	327	Protein_218	transposase	-
NL730_RS25410 (NL730_25410)	188193..188834	+	642	WP_103660514.1	MBL fold metallo-hydrolase	-
NL730_RS25415 (NL730_25415)	189075..189572	-	498	WP_162822358.1	hypothetical protein	-
NL730_RS25420 (NL730_25420)	189657..190460	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
NL730_RS25425 (NL730_25425)	190460..191296	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
NL730_RS25430 (NL730_25430)	191338..191628	-	291	WP_000985910.1	hypothetical protein	-
NL730_RS25435 (NL730_25435)	191730..191936	-	207	WP_000134171.1	hypothetical protein	-
NL730_RS25440 (NL730_25440)	191997..193322	-	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
NL730_RS25445 (NL730_25445)	193327..193620	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
NL730_RS25450 (NL730_25450)	194224..194616	-	393	WP_000341066.1	hypothetical protein	-
NL730_RS25455 (NL730_25455)	194989..195330	+	342	WP_001151575.1	hypothetical protein	-
NL730_RS25460 (NL730_25460)	195422..195613	+	192	WP_000398480.1	hypothetical protein	-
NL730_RS25465 (NL730_25465)	195663..195944	+	282	WP_000077926.1	hypothetical protein	-
NL730_RS25470 (NL730_25470)	196507..197748	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrB4 / blaDHA-1 / sul1 / aph(3')-IIa / floR / aph(4)-Ia / aac(3)-IVa / mph(A) / qacE / aadA2 / dfrA12 / tet(A) / cmlA1 / ant(3'')-Ia / sul3 / aph(3'')-Ib / aph(6)-Id	-	1..294171	294171
	-	flank	IS/Tn	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3'')-Ib / aph(6)-Id	-	179538..191296	11758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T251193 WP_000088046.1 NZ_CP100716:c193322-191997 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT251193 WP_024136327.1 NZ_CP100716:c193620-193327 [Salmonella enterica subsp. enterica serovar Typhimurium]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1