Detailed information of TA system TA025119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 2293961..2294514
• Replicon: chromosome
• Accession: NC_015125
• Organism: Microbacterium testaceum StLB037

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E8NEJ6
• Locus tag: MTES_RS10375
• Protein ID: WP_013585206.1
• Coordinates: 2294242..2294514 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MTES_RS10370
• Protein ID: WP_080575403.1
• Coordinates: 2293961..2294245 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTES_RS10345	2289942..2291207	+	1266	WP_013585201.1	serine--tRNA ligase	-
MTES_RS10350	2291309..2292100	+	792	WP_080575500.1	Cof-type HAD-IIB family hydrolase	-
MTES_RS10355	2292103..2292297	-	195	WP_013585203.1	PLDc N-terminal domain-containing protein	-
MTES_RS10360	2292436..2293701	-	1266	WP_080575402.1	DUF2254 domain-containing protein	-
MTES_RS10370	2293961..2294245	+	285	WP_080575403.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MTES_RS10375	2294242..2294514	+	273	WP_013585206.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MTES_RS10380	2294627..2295922	+	1296	WP_148272851.1	LCP family protein	-
MTES_RS18875	2296498..2296734	+	237	WP_080575501.1	Lsr2 family protein	-
MTES_RS10395	2296791..2298752	-	1962	WP_013585209.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (24-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10302.93 Da
• Isoelectric Point: 10.9215

>T25119 WP_013585206.1 NC_015125:2294242-2294514 [Microbacterium testaceum StLB037]
VTPEYEVVFSRAARRALEQDLPEKVAAAAFEFIAGALRANPRRVGKPLREPLAPLHSARRGDYRVLYRIVDHRLVIEIVS
IVHRRDAYRA

Nucleotide

• Length: 273 bp

>T25119 NC_015125:2294242-2294514 [Microbacterium testaceum StLB037]
GTGACACCCGAGTACGAGGTCGTTTTCAGCCGGGCCGCTCGACGGGCGCTCGAGCAGGATCTCCCCGAGAAGGTCGCGGC
CGCGGCCTTCGAGTTCATCGCGGGCGCCCTCCGCGCCAATCCCCGTCGTGTCGGTAAGCCGCTCCGCGAGCCGCTCGCGC
CGCTCCACTCCGCACGTCGCGGCGACTACCGCGTTCTGTATCGCATCGTCGACCACCGGCTCGTGATCGAGATCGTTTCG
ATCGTTCACCGCCGCGACGCGTACCGAGCCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10182.34 Da
• Isoelectric Point: 4.3837

>AT25119 WP_080575403.1 NC_015125:2293961-2294245 [Microbacterium testaceum StLB037]
MTTLSLADARANLSKLVESAVTTHERFDVTRNGDRVAVLLSADDYDSLLETVDILSRPDEIAAVREGLADLAEGRTSSLE
DVRAAMTVRGRFSS

Nucleotide

• Length: 285 bp

>AT25119 NC_015125:2293961-2294245 [Microbacterium testaceum StLB037]
ATGACCACGCTCTCCCTCGCCGATGCGCGCGCGAACCTCTCGAAGCTCGTCGAGTCCGCCGTCACGACGCACGAGCGGTT
CGATGTCACGCGCAACGGTGACCGCGTCGCCGTCCTTCTGAGCGCCGACGATTACGACTCCCTGCTCGAGACGGTCGACA
TCCTCAGCCGTCCCGACGAGATCGCGGCTGTGCGGGAGGGGCTTGCCGATCTCGCCGAGGGGCGGACCTCGTCCCTCGAG
GATGTTCGTGCGGCGATGACCGTGCGTGGGCGCTTCTCGTCGTGA

Structures

Toxin

• AlphaFold DB: E8NEJ6