Detailed information of TA system TA025114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 480136..480828
• Replicon: chromosome
• Accession: NC_015064
• Organism: Granulicella tundricola MP5ACTX9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E8WXD6
• Locus tag: ACIX9_RS01980
• Protein ID: WP_013578797.1
• Coordinates: 480136..480549 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E8WXD7
• Locus tag: ACIX9_RS01985
• Protein ID: WP_013578798.1
• Coordinates: 480553..480828 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX9_RS01975	477336..480131	+	2796	WP_013578796.1	phosphoenolpyruvate carboxylase	-
ACIX9_RS01980	480136..480549	-	414	WP_013578797.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIX9_RS01985	480553..480828	-	276	WP_013578798.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ACIX9_RS01990	480907..482037	+	1131	WP_013578799.1	A/G-specific adenine glycosylase	-
ACIX9_RS01995	482115..482540	+	426	WP_013578800.1	OsmC family protein	-
ACIX9_RS02000	482901..483713	+	813	WP_157477221.1	hypothetical protein	-
ACIX9_RS02005	483834..484787	-	954	WP_013578802.1	cation diffusion facilitator family transporter	-
ACIX9_RS02010	484849..485205	-	357	WP_013578803.1	four helix bundle protein	-
ACIX9_RS02015	485280..485723	-	444	WP_013578804.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	472815..494857	22042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-131)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14978.45 Da
• Isoelectric Point: 9.6261

>T25114 WP_013578797.1 NC_015064:c480549-480136 [Granulicella tundricola MP5ACTX9]
VLYLLDTDILSQRTKPFPNPQVKAWLAATNDLDTAISVINLLELQQGVGVLPTGKRKKSLEHWLSVSILEIYENRILPIS
PEIALHAGNLVALAKSSGHTATAEDALIAATAHIHALTLVTLNRKHFQRLGAKMLSF

Nucleotide

• Length: 414 bp

>T25114 NC_015064:c480549-480136 [Granulicella tundricola MP5ACTX9]
GTGCTCTATCTCCTTGACACAGACATCCTCTCCCAGCGCACAAAACCATTCCCTAACCCTCAAGTGAAGGCCTGGCTTGC
TGCTACGAACGACTTGGATACTGCGATCAGCGTCATCAATCTGCTGGAACTGCAGCAAGGCGTAGGGGTACTTCCAACCG
GAAAACGCAAGAAAAGCCTTGAGCATTGGCTCTCCGTATCCATCTTGGAGATATACGAAAACCGCATCCTGCCGATCAGT
CCTGAGATAGCGCTCCATGCCGGCAACCTTGTAGCACTCGCGAAATCTTCAGGACACACCGCCACAGCGGAAGACGCCCT
GATAGCCGCAACGGCGCATATCCATGCGCTCACACTGGTGACGCTCAATCGCAAACACTTTCAGCGATTGGGAGCAAAGA
TGCTCTCCTTCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10271.74 Da
• Isoelectric Point: 11.0226

>AT25114 WP_013578798.1 NC_015064:c480828-480553 [Granulicella tundricola MP5ACTX9]
MATWGIAKAKAQLSELIYEAEKTGPQKVTRSGRDVAVVLSIDEWKRLATPDRSETRPQRSVWDVLSNSPLRGSNLKIPRR
RPSTRVIDLSE

Nucleotide

• Length: 276 bp

>AT25114 NC_015064:c480828-480553 [Granulicella tundricola MP5ACTX9]
ATGGCCACATGGGGCATCGCGAAAGCAAAGGCGCAGTTGAGCGAACTGATCTACGAGGCGGAAAAGACCGGCCCGCAGAA
GGTAACGCGCAGTGGCCGGGATGTTGCTGTCGTCCTTTCCATCGACGAATGGAAGCGCCTCGCCACACCTGACCGTTCCG
AAACCAGACCGCAGCGGTCTGTATGGGACGTGCTGAGTAACTCCCCTTTGCGCGGCTCCAATCTCAAAATTCCACGCCGC
AGGCCCAGCACCCGGGTCATCGATCTGAGCGAGTAG

Structures

Toxin

• AlphaFold DB: E8WXD6

Antitoxin

• AlphaFold DB: E8WXD7