Detailed information of TA system TA025108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 282618..284168
• Replicon: plasmid pRAHAQ01
• Accession: NC_015062
• Organism: Rahnella sp. Y9602

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H8P0L7
• Locus tag: RAHAQ_RS23515
• Protein ID: WP_013577980.1
• Coordinates: 282618..283913 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: RAHAQ_RS23520
• Protein ID: WP_013577981.1
• Coordinates: 283917..284168 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RAHAQ_RS23505	278792..279679	+	888	WP_013577978.1	LysR family transcriptional regulator	-
RAHAQ_RS23510	280077..282554	+	2478	WP_013577979.1	membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family	-
RAHAQ_RS23515	282618..283913	-	1296	WP_013577980.1	type II toxin-antitoxin system HipA family toxin	Toxin
RAHAQ_RS23520	283917..284168	-	252	WP_013577981.1	helix-turn-helix domain-containing protein	Antitoxin
RAHAQ_RS23525	284376..285086	+	711	WP_013577982.1	GntR family transcriptional regulator	-
RAHAQ_RS23530	285097..286296	-	1200	WP_013577983.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
RAHAQ_RS23535	286621..288021	+	1401	WP_014416613.1	PTS transporter subunit EIIC	-
RAHAQ_RS23540	288028..288900	-	873	WP_013577985.1	fumarylacetoacetate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaRAHN-2	mgtB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / hcp/tssD / hcp/tssD	1..616549	616549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 48192.43 Da
• Isoelectric Point: 7.0668

>T25108 WP_013577980.1 NC_015062:c283913-282618 [Rahnella sp. Y9602]
MDKLTLALNGIAVGTLEKGGSGEMSFVYHQSWLDRPGARAISLSLPLQADKFRGAVVYNFFDNLLPDNEQIRSRIQARFH
IPTKQPFDLLSRIGGDCVGAIQLYPEGDQVAPVTEIHAQPLGEKDIEQLLQGYKDAPLGMEADIEDFRISLAGAQEKTAL
LWYRDQWHRPQGSTPTSHILKLPIGYIANNGIDLSESVENEWLCLKIAEKFGFPVARSEIASFGAQKVLVVERFDRRWSQ
DGSWLMRLPQEDFCQALGVAPALKYESDGGPGIARAMKLLLGSQRAGADRDMFFKSQILFWMLAAIDGHAKNFSVYIEPG
SAYRMTPFYDVMSAFPLMHLQGIPAKKAKMAMAVLGKNRHFHWDRILPRHFVTTAQRVEYSKENVSAMMTEMKEKTEQVI
ADVEAIIPANFPAKISDAIFAGLRHQAARLP

Nucleotide

• Length: 1296 bp

>T25108 NC_015062:c283913-282618 [Rahnella sp. Y9602]
ATGGATAAGTTAACGCTCGCATTAAATGGCATTGCTGTCGGTACCCTGGAAAAAGGCGGCAGTGGTGAAATGTCATTTGT
TTACCATCAAAGCTGGCTCGATCGCCCCGGAGCCCGCGCCATTTCATTATCATTACCTCTTCAGGCAGATAAATTCCGTG
GCGCAGTGGTATACAACTTTTTTGACAATCTTCTACCCGATAACGAACAGATACGCAGCCGTATTCAGGCACGTTTCCAC
ATCCCGACGAAGCAGCCTTTCGATTTGCTTTCGCGGATCGGTGGTGACTGTGTCGGTGCGATCCAGCTCTATCCTGAAGG
TGATCAGGTTGCACCTGTCACGGAAATTCATGCCCAACCGCTTGGCGAAAAGGATATTGAGCAGCTTCTGCAGGGATATA
AGGATGCACCTTTGGGCATGGAAGCTGACATTGAAGACTTTCGAATTTCTCTGGCCGGTGCACAGGAAAAAACGGCTTTG
CTCTGGTATCGCGACCAGTGGCATCGCCCGCAGGGAAGTACGCCTACCAGCCATATCCTTAAACTTCCTATTGGTTATAT
CGCTAACAATGGCATTGATCTCAGCGAGAGCGTGGAGAATGAATGGTTGTGTCTGAAGATAGCCGAAAAATTTGGATTTC
CTGTTGCACGGTCTGAGATTGCCAGTTTTGGCGCTCAGAAGGTGTTGGTCGTTGAGCGTTTTGACCGCCGCTGGTCGCAG
GATGGATCCTGGCTGATGCGTTTACCTCAGGAAGATTTTTGTCAGGCGCTGGGTGTTGCTCCCGCATTGAAGTATGAATC
TGATGGCGGCCCCGGAATTGCACGCGCAATGAAGCTTTTACTGGGTTCTCAACGGGCGGGGGCGGACAGGGATATGTTCT
TTAAAAGCCAGATCCTCTTCTGGATGCTGGCCGCCATTGACGGTCATGCTAAGAATTTCAGTGTTTATATCGAGCCGGGC
AGCGCATACCGCATGACACCGTTTTATGACGTGATGTCAGCATTTCCTCTGATGCATCTGCAAGGAATTCCGGCGAAGAA
AGCCAAAATGGCGATGGCTGTATTAGGGAAAAACCGGCATTTTCATTGGGACAGAATTCTTCCCCGCCATTTTGTGACGA
CGGCGCAACGGGTCGAGTACTCGAAAGAAAACGTCTCTGCGATGATGACGGAAATGAAGGAAAAAACTGAGCAGGTGATT
GCAGATGTTGAAGCAATAATACCTGCTAATTTTCCGGCAAAAATCAGTGATGCAATTTTTGCAGGCCTGCGCCATCAGGC
TGCACGTTTGCCTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9570.88 Da
• Isoelectric Point: 7.9783

>AT25108 WP_013577981.1 NC_015062:c284168-283917 [Rahnella sp. Y9602]
MNITSAKMLANALRNERKKRKLSQSKTAETIGLKQSTVSEFENYPDGTRLETLFKLLAALDLELQITPRDSNPQEKCTWD
QEW

Nucleotide

• Length: 252 bp

>AT25108 NC_015062:c284168-283917 [Rahnella sp. Y9602]
ATGAACATTACATCTGCAAAAATGCTCGCCAATGCGCTGCGTAATGAGCGAAAAAAGCGCAAACTCAGCCAAAGTAAAAC
GGCTGAAACCATTGGCCTGAAGCAGTCTACGGTTTCTGAGTTCGAAAATTATCCGGACGGTACCCGTCTGGAAACACTGT
TCAAATTATTGGCGGCGCTGGATTTAGAGCTACAGATCACGCCAAGGGATAGCAATCCGCAGGAAAAGTGTACCTGGGAT
CAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: H8P0L7