Detailed information of TA system TA251001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 88676..90299
• Replicon: plasmid pR18.0450_263k
• Accession: NZ_CP100683
• Organism: Salmonella enterica subsp. enterica serovar Goldcoast strain R18.0450

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: FC623_RS22630
• Protein ID: WP_000088046.1
• Coordinates: 88974..90299 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: FC623_RS22625
• Protein ID: WP_024136327.1
• Coordinates: 88676..88969 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FC623_RS22595 (FC623_22595)	84218..84499	-	282	WP_000077926.1	hypothetical protein	-
FC623_RS22600 (FC623_22600)	84549..84740	-	192	WP_000398480.1	hypothetical protein	-
FC623_RS22605 (FC623_22605)	84964..86136	+	1173	WP_000952349.1	IS21-like element IS21 family transposase	-
FC623_RS22610 (FC623_22610)	86136..86933	+	798	WP_000544828.1	IS21-like element IS21 family helper ATPase IstB	-
FC623_RS22615 (FC623_22615)	86972..87307	-	336	WP_065311125.1	hypothetical protein	-
FC623_RS22620 (FC623_22620)	87680..88072	+	393	WP_000341066.1	hypothetical protein	-
FC623_RS22625 (FC623_22625)	88676..88969	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
FC623_RS22630 (FC623_22630)	88974..90299	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
FC623_RS22635 (FC623_22635)	90360..90566	+	207	WP_000134171.1	hypothetical protein	-
FC623_RS22640 (FC623_22640)	90668..91078	+	411	WP_000985911.1	hypothetical protein	-
FC623_RS22645 (FC623_22645)	91091..91906	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
FC623_RS22650 (FC623_22650)	92160..92585	+	426	WP_001043843.1	hypothetical protein	-
FC623_RS22655 (FC623_22655)	93134..93442	+	309	WP_001224687.1	hypothetical protein	-
FC623_RS22660 (FC623_22660)	93458..94315	+	858	WP_000784387.1	HNH endonuclease signature motif containing protein	-
FC623_RS22665 (FC623_22665)	94377..94580	+	204	WP_001194555.1	hypothetical protein	-
FC623_RS22670 (FC623_22670)	94922..95278	-	357	WP_065311124.1	H-NS family nucleoid-associated regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / floR / aph(6)-Id / tet(A) / sul3 / lnu(F) / ant(3'')-Ia / sul2 / aac(3)-IId / blaLAP-2 / blaTEM-1B	-	1..262557	262557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T251001 WP_000088046.1 NZ_CP100683:88974-90299 [Salmonella enterica subsp. enterica serovar Goldcoast]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT251001 WP_024136327.1 NZ_CP100683:88676-88969 [Salmonella enterica subsp. enterica serovar Goldcoast]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1