Detailed information of TA system TA025092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 449490..450076
• Replicon: plasmid pACIX901
• Accession: NC_015057
• Organism: Granulicella tundricola MP5ACTX9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E8X6H6
• Locus tag: ACIX9_RS20340
• Protein ID: WP_013572970.1
• Coordinates: 449699..450076 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E8X6H5
• Locus tag: ACIX9_RS20335
• Protein ID: WP_013572969.1
• Coordinates: 449490..449705 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX9_RS20320	446818..448455	+	1638	WP_013572967.1	alpha-L-fucosidase	-
ACIX9_RS25070	448517..448924	+	408	Protein_285	IS30 family transposase	-
ACIX9_RS20330	448998..449396	-	399	WP_013572968.1	DUF1801 domain-containing protein	-
ACIX9_RS20335	449490..449705	+	216	WP_013572969.1	ribbon-helix-helix protein, CopG family	Antitoxin
ACIX9_RS20340	449699..450076	+	378	WP_013572970.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIX9_RS20345	450537..452990	-	2454	WP_013572971.1	hypothetical protein	-
ACIX9_RS20350	453062..453586	+	525	WP_013572972.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..475658	475658
	-	flank	IS/Tn	-	-	448664..448924	260

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.29 Da
• Isoelectric Point: 8.5812

>T25092 WP_013572970.1 NC_015057:449699-450076 [Granulicella tundricola MP5ACTX9]
MVAVPSLYDTNILIDYLNGLEAARTELESDVAQDRAISAITWMEVMVGVPQSLEAPVRRFLSTFKHLPVTRAVSEEAFTI
RRQSRVKLPDAIMLATARAHNRVLVTRNTRDFPVQTIGFHVPYTL

Nucleotide

• Length: 378 bp

>T25092 NC_015057:449699-450076 [Granulicella tundricola MP5ACTX9]
ATGGTAGCCGTCCCCTCTCTCTACGACACCAATATCCTGATCGACTATCTCAACGGTCTCGAAGCTGCCAGAACCGAACT
GGAATCTGACGTCGCCCAGGACCGAGCCATCAGCGCCATCACCTGGATGGAGGTCATGGTTGGCGTTCCGCAATCCTTGG
AAGCGCCGGTTCGGAGATTTCTCTCCACCTTCAAACATCTCCCCGTCACCCGCGCTGTCTCAGAGGAAGCCTTCACAATT
CGCAGGCAAAGCAGGGTGAAACTACCTGATGCCATCATGCTTGCGACCGCCCGCGCCCACAACCGCGTCCTGGTCACTCG
CAACACGCGTGATTTCCCCGTGCAAACGATAGGTTTCCACGTTCCTTATACGCTTTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7934.00 Da
• Isoelectric Point: 5.0349

>AT25092 WP_013572969.1 NC_015057:449490-449705 [Granulicella tundricola MP5ACTX9]
MRTLIDIPEEDIAAIKQVTVALGISRAEFVRRAVSQSLEPYRTSGHSEAFGICAGKLEDGLQFQDRLRGEW

Nucleotide

• Length: 216 bp

>AT25092 NC_015057:449490-449705 [Granulicella tundricola MP5ACTX9]
ATGCGGACCCTCATCGACATCCCCGAAGAAGACATTGCAGCCATCAAGCAAGTCACAGTGGCCCTCGGGATCTCACGCGC
TGAATTCGTCCGCCGCGCCGTCTCACAGTCTCTTGAGCCCTACCGAACCTCTGGTCACTCGGAAGCCTTCGGAATCTGCG
CGGGAAAACTTGAGGACGGTCTCCAATTCCAGGATCGGCTGCGCGGTGAATGGTAG

Structures

Toxin

• AlphaFold DB: E8X6H6

Antitoxin

• AlphaFold DB: E8X6H5