250903

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4928180..4928820 Replicon chromosome
Accession NZ_CP100652
Organism Pseudomonas putida strain HSM-C2

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag NL778_RS22440 Protein ID WP_012316423.1
Coordinates 4928410..4928820 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A427FTN1
Locus tag NL778_RS22435 Protein ID WP_012316422.1
Coordinates 4928180..4928410 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL778_RS22415 (NL778_22415) 4923567..4924883 - 1317 WP_254466626.1 precorrin-3B synthase -
NL778_RS22420 (NL778_22420) 4925074..4926285 + 1212 WP_042111775.1 bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase -
NL778_RS22425 (NL778_22425) 4926278..4927372 + 1095 WP_012316420.1 cobalt-precorrin-5B (C(1))-methyltransferase -
NL778_RS22430 (NL778_22430) 4927369..4928088 + 720 WP_254466627.1 cobalt-precorrin-6A reductase -
NL778_RS22435 (NL778_22435) 4928180..4928410 + 231 WP_012316422.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NL778_RS22440 (NL778_22440) 4928410..4928820 + 411 WP_012316423.1 tRNA(fMet)-specific endonuclease VapC Toxin
NL778_RS22445 (NL778_22445) 4928955..4929392 + 438 WP_012316424.1 NfeD family protein -
NL778_RS22450 (NL778_22450) 4929417..4930271 + 855 WP_012316425.1 SPFH domain-containing protein -
NL778_RS22455 (NL778_22455) 4930465..4930854 + 390 WP_012316426.1 DUF2946 domain-containing protein -
NL778_RS22460 (NL778_22460) 4930903..4931382 + 480 WP_254466629.1 copper chaperone PCu(A)C -
NL778_RS22465 (NL778_22465) 4931409..4931840 + 432 WP_012316428.1 DUF2946 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15055.42 Da        Isoelectric Point: 8.0998

>T250903 WP_012316423.1 NZ_CP100652:4928410-4928820 [Pseudomonas putida]
MLKYMLDTNICIFTIKNKPQVVREAFNRHHGQLAISTVTLMELVYGAEKSAAPARNLSIVEGFVARLEVLDYDSHAAEHS
GQLRSELAKAGTPIGPFDQLIAGHARARGLTLVTNNLREFMRVPGLRVEDWLAMPG

Download         Length: 411 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8487.35 Da        Isoelectric Point: 4.2821

>AT250903 WP_012316422.1 NZ_CP100652:4928180-4928410 [Pseudomonas putida]
MEQGAVFKSNRSQAIRLPKSVAFPEEVTRVDIVAVGRTRIITPAGESWDSWFDGEDASPDFMASRDQPADQEREGF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A427FTN1

References