Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 523113..523749 Replicon chromosome
Accession NZ_CP100651
Organism Bacillus halotolerans strain MEC_B334

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NLW79_RS02655 Protein ID WP_003156187.1
Coordinates 523399..523749 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NLW79_RS02650 Protein ID WP_003225183.1
Coordinates 523113..523394 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLW79_RS02630 (519468) 519468..520067 - 600 WP_254518054.1 rhomboid family intramembrane serine protease -
NLW79_RS02635 (520162) 520162..520527 + 366 WP_106020948.1 holo-ACP synthase -
NLW79_RS02640 (520693) 520693..521709 + 1017 WP_106021112.1 outer membrane lipoprotein carrier protein LolA -
NLW79_RS02645 (521826) 521826..522995 + 1170 WP_024120297.1 alanine racemase -
NLW79_RS02650 (523113) 523113..523394 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NLW79_RS02655 (523399) 523399..523749 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NLW79_RS02660 (523865) 523865..524689 + 825 WP_081638235.1 RsbT co-antagonist protein RsbRA -
NLW79_RS02665 (524694) 524694..525059 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NLW79_RS02670 (525062) 525062..525463 + 402 WP_106020946.1 serine/threonine-protein kinase RsbT -
NLW79_RS02675 (525475) 525475..526482 + 1008 WP_024120300.1 phosphoserine phosphatase RsbU -
NLW79_RS02680 (526543) 526543..526872 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
NLW79_RS02685 (526869) 526869..527351 + 483 WP_024120302.1 anti-sigma B factor RsbW -
NLW79_RS02690 (527317) 527317..528105 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
NLW79_RS02695 (528105) 528105..528704 + 600 WP_024120304.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T250891 WP_003156187.1 NZ_CP100651:523399-523749 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT250891 WP_003225183.1 NZ_CP100651:523113-523394 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References