Detailed information of TA system TA025089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1041160..1041793
• Replicon: chromosome
• Accession: NC_015052
• Organism: Bifidobacterium longum subsp. infantis 157F

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EYF0
• Locus tag: BLIF_RS04345
• Protein ID: WP_012577854.1
• Coordinates: 1041425..1041793 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: E8MT91
• Locus tag: BLIF_RS04340
• Protein ID: WP_015713502.1
• Coordinates: 1041160..1041444 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLIF_RS04280	1036291..1036518	+	228	WP_041473841.1	helix-turn-helix domain-containing protein	-
BLIF_RS04285	1036669..1036935	+	267	WP_015713493.1	hypothetical protein	-
BLIF_RS04290	1036949..1037218	+	270	WP_015713494.1	hypothetical protein	-
BLIF_RS10650	1037411..1037599	+	189	WP_032742291.1	helix-turn-helix domain-containing protein	-
BLIF_RS04305	1038017..1038445	+	429	WP_148235523.1	hypothetical protein	-
BLIF_RS04310	1038442..1038786	+	345	WP_015713496.1	hypothetical protein	-
BLIF_RS04315	1038899..1039246	+	348	WP_015713497.1	hypothetical protein	-
BLIF_RS04320	1039441..1040187	+	747	WP_015713498.1	hypothetical protein	-
BLIF_RS04325	1040189..1040518	+	330	WP_015713499.1	hypothetical protein	-
BLIF_RS04330	1040518..1040700	+	183	WP_015713500.1	hypothetical protein	-
BLIF_RS04335	1040786..1041088	+	303	WP_015713501.1	hypothetical protein	-
BLIF_RS04340	1041160..1041444	+	285	WP_015713502.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLIF_RS04345	1041425..1041793	+	369	WP_012577854.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLIF_RS04350	1041980..1042243	+	264	WP_015713504.1	hypothetical protein	-
BLIF_RS04355	1042240..1042467	+	228	WP_015713505.1	hypothetical protein	-
BLIF_RS04360	1042464..1043219	+	756	WP_015713506.1	hypothetical protein	-
BLIF_RS04365	1043328..1043576	+	249	WP_015713507.1	hypothetical protein	-
BLIF_RS04370	1043738..1044004	-	267	WP_041473844.1	hypothetical protein	-
BLIF_RS04375	1044001..1044222	-	222	WP_015713509.1	hypothetical protein	-
BLIF_RS04380	1044302..1045519	-	1218	WP_015713510.1	transposase	-
BLIF_RS04385	1045675..1046163	-	489	WP_015713511.1	hypothetical protein	-
BLIF_RS04390	1046322..1046579	-	258	WP_012577847.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1006192..1068219	62027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (16-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-53)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13866.75 Da
• Isoelectric Point: 4.5746

>T25089 WP_012577854.1 NC_015052:1041425-1041793 [Bifidobacterium longum subsp. infantis 157F]
MTSTPSEPRLYDVWLMWVEFPDHPGIGKPRPVVITEVDGDLVSGIVAKITGNTDWDEAGDVPLLDWKAEGLLKPSLVRCS
QRFYFNRSELLQWFGRLSLRDAEHVNDGLEATLDIPPYRRSV

Nucleotide

• Length: 369 bp

>T25089 NC_015052:1041425-1041793 [Bifidobacterium longum subsp. infantis 157F]
ATGACCAGTACGCCAAGTGAACCGCGCCTGTATGACGTGTGGCTGATGTGGGTCGAGTTTCCCGACCATCCCGGTATCGG
GAAGCCGCGTCCGGTGGTTATCACCGAGGTTGACGGTGATCTGGTGTCGGGTATCGTGGCGAAGATAACCGGCAACACTG
ATTGGGATGAGGCCGGTGACGTGCCGCTGCTCGACTGGAAGGCCGAGGGGCTGTTGAAGCCGTCACTCGTGCGCTGTTCG
CAACGCTTCTACTTCAACAGGAGCGAACTGCTGCAATGGTTCGGACGACTCTCGTTGAGGGACGCGGAGCATGTTAACGA
CGGATTGGAAGCCACGTTGGACATTCCACCATACAGGCGGAGCGTATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10531.82 Da
• Isoelectric Point: 4.5582

>AT25089 WP_015713502.1 NC_015052:1041160-1041444 [Bifidobacterium longum subsp. infantis 157F]
MGKLVANIDDDVKARAAALYDSMGMSLSTAVNMFLRQSLVDNGLPFKPTRHTPDGYPVPPVHNAYMFERSEKGHVILPAD
WDDSEDDIYDQYAK

Nucleotide

• Length: 285 bp

>AT25089 NC_015052:1041160-1041444 [Bifidobacterium longum subsp. infantis 157F]
ATGGGTAAGCTGGTCGCCAATATCGATGATGATGTCAAGGCGCGTGCCGCCGCGCTCTACGATTCCATGGGCATGAGCCT
GAGCACCGCCGTCAACATGTTCCTACGCCAGTCTCTGGTGGACAACGGGTTGCCGTTCAAGCCGACGCGACACACGCCGG
ACGGTTATCCGGTGCCGCCTGTTCACAATGCATACATGTTCGAGCGTTCGGAGAAGGGCCATGTGATACTGCCCGCCGAT
TGGGATGATTCGGAGGATGATATCTATGACCAGTACGCCAAGTGA

Structures

Toxin

• AlphaFold DB: W6EYF0

Antitoxin

• AlphaFold DB: E8MT91