Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5186878..5187503 Replicon chromosome
Accession NZ_CP100601
Organism Klebsiella pneumoniae strain WX17388

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NLI98_RS25050 Protein ID WP_002882817.1
Coordinates 5186878..5187261 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NLI98_RS25055 Protein ID WP_004150355.1
Coordinates 5187261..5187503 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLI98_RS25035 (5184244) 5184244..5185146 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NLI98_RS25040 (5185143) 5185143..5185778 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NLI98_RS25045 (5185775) 5185775..5186704 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NLI98_RS25050 (5186878) 5186878..5187261 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NLI98_RS25055 (5187261) 5187261..5187503 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NLI98_RS25060 (5187708) 5187708..5188625 + 918 WP_088497967.1 alpha/beta hydrolase -
NLI98_RS25065 (5188639) 5188639..5189580 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NLI98_RS25070 (5189625) 5189625..5190062 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NLI98_RS25075 (5190059) 5190059..5190919 - 861 WP_264452046.1 virulence factor BrkB family protein -
NLI98_RS25080 (5190913) 5190913..5191512 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250885 WP_002882817.1 NZ_CP100601:c5187261-5186878 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250885 WP_004150355.1 NZ_CP100601:c5187503-5187261 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References