Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4588679..4589489 Replicon chromosome
Accession NZ_CP100601
Organism Klebsiella pneumoniae strain WX17388

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NLI98_RS22225 Protein ID WP_004178461.1
Coordinates 4588679..4589212 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NLI98_RS22230 Protein ID WP_002887278.1
Coordinates 4589223..4589489 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLI98_RS22220 (4587510) 4587510..4588631 + 1122 WP_065807264.1 cupin domain-containing protein -
NLI98_RS22225 (4588679) 4588679..4589212 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NLI98_RS22230 (4589223) 4589223..4589489 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NLI98_RS22235 (4589592) 4589592..4591025 - 1434 WP_016946714.1 Cu(+)/Ag(+) sensor histidine kinase -
NLI98_RS22240 (4591015) 4591015..4591698 - 684 WP_065807266.1 copper response regulator transcription factor CusR -
NLI98_RS22245 (4591870) 4591870..4593255 + 1386 WP_004177562.1 efflux transporter outer membrane subunit -
NLI98_RS22250 (4593273) 4593273..4593617 + 345 WP_088498161.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T250883 WP_004178461.1 NZ_CP100601:c4589212-4588679 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT250883 WP_002887278.1 NZ_CP100601:c4589489-4589223 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References