Detailed information of TA system TA025087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2733061..2733697
• Replicon: chromosome
• Accession: NC_014976
• Organism: Bacillus subtilis BSn5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BSN5_RS14225
• Protein ID: WP_003156187.1
• Coordinates: 2733347..2733697 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: BSN5_RS14220
• Protein ID: WP_003225183.1
• Coordinates: 2733061..2733342 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSN5_RS14200	2729420..2730019	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
BSN5_RS14205	2730114..2730479	+	366	WP_003234281.1	holo-ACP synthase	-
BSN5_RS14210	2730645..2731661	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
BSN5_RS14215	2731776..2732945	+	1170	WP_003234284.1	alanine racemase	-
BSN5_RS14220	2733061..2733342	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BSN5_RS14225	2733347..2733697	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BSN5_RS14230	2733812..2734636	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
BSN5_RS14235	2734641..2735006	+	366	WP_015715277.1	RsbT antagonist protein RsbS	-
BSN5_RS14240	2735010..2735411	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
BSN5_RS14245	2735423..2736430	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
BSN5_RS14250	2736492..2736821	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
BSN5_RS14255	2736818..2737300	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
BSN5_RS14260	2737266..2738054	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
BSN5_RS14265	2738054..2738653	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T25087 WP_003156187.1 NC_014976:2733347-2733697 [Bacillus subtilis BSn5]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T25087 NC_014976:2733347-2733697 [Bacillus subtilis BSn5]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT25087 WP_003225183.1 NC_014976:2733061-2733342 [Bacillus subtilis BSn5]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT25087 NC_014976:2733061-2733342 [Bacillus subtilis BSn5]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32