Detailed information of TA system TA250854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 4126709..4128121
• Replicon: chromosome
• Accession: NZ_CP100553
• Organism: Pseudomonas hydrolytica strain KHPS2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: NLY38_RS19400
• Protein ID: WP_096825807.1
• Coordinates: 4127552..4128121 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: NLY38_RS19395
• Protein ID: WP_195881883.1
• Coordinates: 4126709..4127548 (-)
• Length: 280 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NLY38_RS19380 (NLY38_19380)	4123626..4124531	-	906	WP_195881885.1	GIDE domain-containing protein	-
NLY38_RS19385 (NLY38_19385)	4124533..4125105	-	573	WP_003246031.1	LemA family protein	-
NLY38_RS19390 (NLY38_19390)	4125296..4126633	+	1338	WP_195881884.1	imelysin family protein	-
NLY38_RS19395 (NLY38_19395)	4126709..4127548	-	840	WP_195881883.1	AraC family transcriptional regulator	Antitoxin
NLY38_RS19400 (NLY38_19400)	4127552..4128121	-	570	WP_096825807.1	GNAT family protein	Toxin
NLY38_RS19405 (NLY38_19405)	4128322..4131810	+	3489	WP_148118800.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
NLY38_RS19410 (NLY38_19410)	4131853..4132671	-	819	WP_195881882.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• DNA binding HTH domain, AraC-type (197-274)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21341.51 Da
• Isoelectric Point: 9.8676

>T250854 WP_096825807.1 NZ_CP100553:c4128121-4127552 [Pseudomonas hydrolytica]
MFKPQLVTLQRGALRLEPLADADIPALVALAEANRDELLYMSGTQRLDWYRSALAELREQRALPFAIRLGEQLVGTTRFG
DFMNSLPACEIGWTWLDRAQHGSGLNASIKYLMLKHAFDNWGMVRVQLKTAASNLRSQRAIEKLGAAREGLLRNHRRLAD
GRLDHTVLYSITDQEWPQVRAALEARFGG

Nucleotide

• Length: 570 bp

Antitoxin

Protein

• Length: 280 a.a.
• Molecular weight: 30928.05 Da
• Isoelectric Point: 7.7422

>AT250854 WP_195881883.1 NZ_CP100553:c4127548-4126709 [Pseudomonas hydrolytica]
MQPLTGSERARFSASDDLHGLELLSARFVEHRFAPHVHDGHVIAVLEAGAERYRYRGVDHLAGAGSIALLNPDEVHTGSK
GVEQGWQYRVFYPQPGTFEALLAELESPPAAPPMFHDSVHHDPLLASRLSALHRLLEDPHSEALQRQTLWREAMLELLQR
YARLPAPRGAGQEPRAVALAKELLASQLSEPPSLEELAAAVNLSPFHFARVFRRATGLPPHAWLRQRRLEQARTLLRSGC
SALSVAMQLGFADQSHLSRQFKQTYGVSPGEYRSAVARG

Nucleotide

• Length: 840 bp