250835

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3458241..3459078 Replicon chromosome
Accession NZ_CP100544
Organism Escherichia coli strain LH09-a

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NLZ06_RS16680 Protein ID WP_000227784.1
Coordinates 3458536..3459078 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NLZ06_RS16675 Protein ID WP_001297137.1
Coordinates 3458241..3458552 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ06_RS16650 (3453261) 3453261..3454208 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
NLZ06_RS16655 (3454230) 3454230..3456221 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NLZ06_RS16660 (3456211) 3456211..3456825 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NLZ06_RS16665 (3456825) 3456825..3457154 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NLZ06_RS16670 (3457166) 3457166..3458056 + 891 WP_000971336.1 heme o synthase -
NLZ06_RS16675 (3458241) 3458241..3458552 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NLZ06_RS16680 (3458536) 3458536..3459078 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NLZ06_RS16685 (3459134) 3459134..3460069 - 936 WP_001365761.1 tetratricopeptide repeat protein -
NLZ06_RS16690 (3460477) 3460477..3461841 + 1365 WP_001000975.1 MFS transporter -
NLZ06_RS16695 (3461969) 3461969..3462460 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NLZ06_RS16700 (3462628) 3462628..3463539 + 912 WP_000705877.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T250835 WP_000227784.1 NZ_CP100544:3458536-3459078 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT250835 WP_001297137.1 NZ_CP100544:3458241-3458552 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References