250826

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 984220..984803 Replicon chromosome
Accession NZ_CP100544
Organism Escherichia coli strain LH09-a

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag NLZ06_RS04755 Protein ID WP_000254745.1
Coordinates 984468..984803 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NLZ06_RS04750 Protein ID WP_000581937.1
Coordinates 984220..984468 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ06_RS04740 (980559) 980559..981860 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
NLZ06_RS04745 (981908) 981908..984142 + 2235 WP_000226828.1 GTP diphosphokinase -
NLZ06_RS04750 (984220) 984220..984468 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NLZ06_RS04755 (984468) 984468..984803 + 336 WP_000254745.1 endoribonuclease MazF Toxin
NLZ06_RS04760 (984874) 984874..985665 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NLZ06_RS04765 (985893) 985893..987530 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NLZ06_RS04770 (987618) 987618..988916 + 1299 WP_000036723.1 phosphopyruvate hydratase -
NLZ06_RS04775 (988972) 988972..989334 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T250826 WP_000254745.1 NZ_CP100544:984468-984803 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT250826 WP_000581937.1 NZ_CP100544:984220-984468 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References