Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 285516..286316 Replicon chromosome
Accession NZ_CP100544
Organism Escherichia coli strain LH09-a

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NLZ06_RS01330 Protein ID WP_000342449.1
Coordinates 285789..286316 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NLZ06_RS01325 Protein ID WP_001277108.1
Coordinates 285516..285782 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ06_RS01305 (281174) 281174..281842 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NLZ06_RS01310 (281835) 281835..282893 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NLZ06_RS01315 (283138) 283138..283992 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NLZ06_RS01320 (284263) 284263..285366 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NLZ06_RS01325 (285516) 285516..285782 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NLZ06_RS01330 (285789) 285789..286316 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NLZ06_RS01335 (286313) 286313..286696 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
NLZ06_RS01340 (287120) 287120..288229 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NLZ06_RS01345 (288277) 288277..289203 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NLZ06_RS01350 (289200) 289200..290477 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
NLZ06_RS01355 (290474) 290474..291241 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T250823 WP_000342449.1 NZ_CP100544:285789-286316 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT250823 WP_001277108.1 NZ_CP100544:285516-285782 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References