Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5113754..5114379 Replicon chromosome
Accession NZ_CP100542
Organism Klebsiella pneumoniae strain LH09-d

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NLZ07_RS24965 Protein ID WP_002882817.1
Coordinates 5113754..5114137 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NLZ07_RS24970 Protein ID WP_004150355.1
Coordinates 5114137..5114379 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ07_RS24950 (5111120) 5111120..5112022 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NLZ07_RS24955 (5112019) 5112019..5112654 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NLZ07_RS24960 (5112651) 5112651..5113580 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NLZ07_RS24965 (5113754) 5113754..5114137 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NLZ07_RS24970 (5114137) 5114137..5114379 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NLZ07_RS24975 (5114584) 5114584..5115501 + 918 WP_023302328.1 alpha/beta hydrolase -
NLZ07_RS24980 (5115515) 5115515..5116456 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NLZ07_RS24985 (5116501) 5116501..5116938 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NLZ07_RS24990 (5116935) 5116935..5117795 - 861 WP_004146232.1 virulence factor BrkB family protein -
NLZ07_RS24995 (5117789) 5117789..5118388 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T250821 WP_002882817.1 NZ_CP100542:c5114137-5113754 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT250821 WP_004150355.1 NZ_CP100542:c5114379-5114137 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References