Detailed information of TA system TA025081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 1533402..1534168
• Replicon: chromosome
• Accession: NC_014965
• Organism: Vibrio vulnificus MO6-24/O

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VVMO6_RS07145
• Protein ID: WP_013571702.1
• Coordinates: 1533402..1533899 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A3N3DV16
• Locus tag: VVMO6_RS07150
• Protein ID: WP_013571703.1
• Coordinates: 1533896..1534168 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVMO6_RS23100	1528717..1528845	+	129	WP_072612488.1	DUF3265 domain-containing protein	-
VVMO6_RS07110	1528873..1529328	+	456	WP_005464666.1	GNAT family N-acetyltransferase	-
VVMO6_RS23105	1529325..1529447	+	123	WP_072612498.1	DUF3265 domain-containing protein	-
VVMO6_RS07115	1529467..1529874	+	408	WP_010644697.1	NUDIX hydrolase	-
VVMO6_RS23110	1529881..1530005	+	125	Protein_1424	DUF3265 domain-containing protein	-
VVMO6_RS07120	1530022..1530543	+	522	WP_000058623.1	GrpB family protein	-
VVMO6_RS22525	1530679..1531290	+	612	WP_052299107.1	HNH endonuclease	-
VVMO6_RS23120	1531757..1531924	+	168	WP_072612489.1	DUF3265 domain-containing protein	-
VVMO6_RS07130	1531911..1532270	+	360	WP_013571698.1	VOC family protein	-
VVMO6_RS07135	1532419..1532706	+	288	WP_013571699.1	hypothetical protein	-
VVMO6_RS07140	1532883..1533161	-	279	WP_044023864.1	hypothetical protein	-
VVMO6_RS07145	1533402..1533899	-	498	WP_013571702.1	GNAT family N-acetyltransferase	Toxin
VVMO6_RS07150	1533896..1534168	-	273	WP_013571703.1	DUF1778 domain-containing protein	Antitoxin
VVMO6_RS24200	1534278..1534370	+	93	WP_005433818.1	DUF3265 domain-containing protein	-
VVMO6_RS24155	1534418..1534762	-	345	WP_162470134.1	hypothetical protein	-
VVMO6_RS23135	1534863..1534979	+	117	WP_102970002.1	DUF3265 domain-containing protein	-
VVMO6_RS07160	1535016..1535612	+	597	WP_013571707.1	class I SAM-dependent methyltransferase	-
VVMO6_RS23140	1535609..1535749	+	141	Protein_1437	DUF3265 domain-containing protein	-
VVMO6_RS07165	1535743..1536123	+	381	WP_029796920.1	hypothetical protein	-
VVMO6_RS23145	1536599..1536748	+	150	WP_072612490.1	DUF3265 domain-containing protein	-
VVMO6_RS23155	1537087..1537209	+	123	WP_072612152.1	DUF3265 domain-containing protein	-
VVMO6_RS07170	1537250..1537603	+	354	WP_013571710.1	hypothetical protein	-
VVMO6_RS07175	1537634..1538194	+	561	WP_175546619.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flp1 / VP1611	1185032..1724682	539650
	-	inside	Integron	-	-	1434218..1558944	124726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18428.09 Da
• Isoelectric Point: 8.8085

>T25081 WP_013571702.1 NC_014965:c1533899-1533402 [Vibrio vulnificus MO6-24/O]
MMNTVLLDKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNDNSHVIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKGGAKHFYERYGFQAFEDAENKLFITIADV
RASLG

Nucleotide

• Length: 498 bp

>T25081 NC_014965:c1533899-1533402 [Vibrio vulnificus MO6-24/O]
ATGATGAATACGGTACTTCTAGATAAAGCTAAACACGATAGAAACCGATTCAACTGTGGTATCGAAGCTCTCAATAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCAAAAAAAGACAACACCAGAACATTTGTTTTGGAAGATGATAACGACAACTCAC
ATGTCATCGGCTTTTACACGCTAACAATGACACCAATTGACTTAAAGGCATTGCCCGATAAGTTACAAAAAAAACACCAA
TCATCCACCTCTGGTGGTCTGATTGCCCGCCTTGCTGTCGATGACCGATACAAAGGGAAAGGCTTTGGTGAGTGGCTGCT
TATTGACGCACTCAGAAAGCTATTAGCTGCAAGTGATAGCGTTGCATTTCCAGTTGTTATCGTTGATGCCAAAGGCGGTG
CAAAACACTTCTATGAACGCTATGGTTTTCAAGCATTTGAAGACGCTGAAAATAAACTCTTCATCACCATTGCTGATGTG
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9747.28 Da
• Isoelectric Point: 8.5960

>AT25081 WP_013571703.1 NC_014965:c1534168-1533896 [Vibrio vulnificus MO6-24/O]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMEALDRPATQNSKLKA
AADRYESKIQ

Nucleotide

• Length: 273 bp

>AT25081 NC_014965:c1534168-1533896 [Vibrio vulnificus MO6-24/O]
ATGGCTACTACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACACAAGACTTACTTACTAAGGCTGCCGCGATCGC
CGGTATGTCTAGCATCAACTCGTTCGTTCTAAGCGCTGCAATCGAAAAAGCTAAACAAGTCATCGAACGTGAACAGGCTT
TAAAACTGAGCCAAGCAGATGCGATGTTGCTCATGGAAGCTTTAGACCGTCCTGCAACACAGAACTCAAAACTAAAAGCT
GCTGCTGATCGATACGAGAGCAAAATTCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3N3DV16