250792

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3674421..3675258 Replicon chromosome
Accession NZ_CP100530
Organism Escherichia coli strain LH10-b-1

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag NLZ08_RS18175 Protein ID WP_000227786.1
Coordinates 3674716..3675258 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NLZ08_RS18170 Protein ID WP_001297137.1
Coordinates 3674421..3674732 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ08_RS18145 (3669441) 3669441..3670388 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
NLZ08_RS18150 (3670410) 3670410..3672401 + 1992 WP_254547525.1 cytochrome o ubiquinol oxidase subunit I -
NLZ08_RS18155 (3672391) 3672391..3673005 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NLZ08_RS18160 (3673005) 3673005..3673334 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NLZ08_RS18165 (3673346) 3673346..3674236 + 891 WP_000971336.1 heme o synthase -
NLZ08_RS18170 (3674421) 3674421..3674732 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NLZ08_RS18175 (3674716) 3674716..3675258 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
NLZ08_RS18180 (3675314) 3675314..3676249 - 936 WP_001297127.1 tetratricopeptide repeat protein -
NLZ08_RS18185 (3676666) 3676666..3678030 + 1365 WP_001000978.1 MFS transporter -
NLZ08_RS18190 (3678158) 3678158..3678649 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NLZ08_RS18195 (3678817) 3678817..3679728 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T250792 WP_000227786.1 NZ_CP100530:3674716-3675258 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT250792 WP_001297137.1 NZ_CP100530:3674421-3674732 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References