Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2457329..2458917 Replicon chromosome
Accession NZ_CP100530
Organism Escherichia coli strain LH10-b-1

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag NLZ08_RS12005 Protein ID WP_254547499.1
Coordinates 2457595..2458917 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag NLZ08_RS12000 Protein ID WP_001301023.1
Coordinates 2457329..2457595 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ08_RS11975 (2452504) 2452504..2452794 - 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -
NLZ08_RS11980 (2452818) 2452818..2453693 - 876 WP_097749322.1 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
NLZ08_RS11985 (2453720) 2453720..2454742 - 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
NLZ08_RS11990 (2454754) 2454754..2455530 - 777 Protein_2350 histidine kinase -
NLZ08_RS11995 (2455526) 2455526..2457106 + 1581 Protein_2351 autotransporter outer membrane beta-barrel domain-containing protein -
NLZ08_RS12000 (2457329) 2457329..2457595 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
NLZ08_RS12005 (2457595) 2457595..2458917 + 1323 WP_254547499.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
NLZ08_RS12010 (2459289) 2459289..2459771 + 483 WP_000931227.1 fimbrial protein -
NLZ08_RS12015 (2460132) 2460132..2460842 + 711 WP_254547500.1 fimbria/pilus periplasmic chaperone -
NLZ08_RS12020 (2460884) 2460884..2463534 + 2651 Protein_2356 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element qnrS1 / blaSHV-12 sodB / espR1 / espR1 2310445..2635036 324591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49241.80 Da        Isoelectric Point: 8.2482

>T250789 WP_254547499.1 NZ_CP100530:2457595-2458917 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDLPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT250789 WP_001301023.1 NZ_CP100530:2457329-2457595 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References