Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 953242..953896 Replicon chromosome
Accession NZ_CP100530
Organism Escherichia coli strain LH10-b-1

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag NLZ08_RS04620 Protein ID WP_000244783.1
Coordinates 953489..953896 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NLZ08_RS04615 Protein ID WP_000354046.1
Coordinates 953242..953508 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NLZ08_RS04590 (948411) 948411..949154 + 744 WP_097749237.1 SDR family oxidoreductase -
NLZ08_RS04595 (949211) 949211..950644 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
NLZ08_RS04600 (950689) 950689..951000 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NLZ08_RS04605 (951164) 951164..951823 + 660 WP_000250274.1 hemolysin III family protein -
NLZ08_RS04610 (952019) 952019..952999 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NLZ08_RS04615 (953242) 953242..953508 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NLZ08_RS04620 (953489) 953489..953896 + 408 WP_000244783.1 protein YgfX Toxin
NLZ08_RS04625 (953936) 953936..954457 - 522 WP_001055874.1 flavodoxin FldB -
NLZ08_RS04630 (954569) 954569..955465 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NLZ08_RS04635 (955490) 955490..956200 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NLZ08_RS04640 (956206) 956206..957939 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T250781 WP_000244783.1 NZ_CP100530:953489-953896 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT250781 WP_000354046.1 NZ_CP100530:953242-953508 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References